Salehi-Nik Nasim, Malaie-Balasi Zahra, Amoabediny Ghassem, Banikarimi Seyedeh Parnian, Zandieh-Doulabi Behrouz, Klein-Nulend Jenneke
School of Chemical Engineering, College of Engineering, University of Tehran,111554563, Enqelab Avenue, Tehran, Iran; Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, MOVE Research Institute Amsterdam, Gustav Mahlerlaan 3008, 1081 LA Amsterdam, The Netherlands; Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, 1985717443, Velenjak, Shahid Chamran Highway, Tehran, Iran.
Research Center for New Technologies in Life Science Engineering, University of Tehran,143951374, Enqelab Avenue, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2017 Aug 1;77:1204-1215. doi: 10.1016/j.msec.2017.03.172. Epub 2017 Mar 21.
Biocompatibility of biomedical devices can be improved by endothelialization of blood-contacting parts mimicking the vascular endothelium's function. Improved endothelialization might be obtained by using biomimetic coatings that allow local sustained release of biologically active molecules, e.g. anti-thrombotic and growth-inducing agents, from nanoliposomes. We aimed to test whether incorporation of growth-inducing nanoliposomal growth hormone (nGH) and anti-thrombotic nanoliposomal sodium nitrite (nNitrite) into collagen coating of silicone tubes enhances endothelialization by stimulating endothelial cell proliferation and inhibiting platelet adhesion. Collagen coating stably immobilized on acrylic acid-grafted silicone tubes decreased the water contact angle from 102° to 56°. Incorporation of 50 or 500nmol/ml nNitrite and 100 or 1000ng/ml nGH into collagen coating decreased the water contact angle further to 48°. After 120h incubation, 58% nitrite and 22% GH of the initial amount of sodium nitrite and GH in nanoliposomes were gradually released from the nNitrite-nGH-collagen coating. Endothelial cell number was increased after surface coating of silicone tubes with collagen by 1.6-fold, and with nNitrite-nGH-collagen conjugate by 1.8-3.9-fold after 2days. After 6days, endothelial cell confluency in the absence of surface coating was 22%, with collagen coating 74%, and with nNitrite-nGH-collagen conjugate coating 83-119%. In the absence of endothelial cells, platelet adhesion was stimulated after collagen coating by 1.3-fold, but inhibited after nNitrite-nGH-collagen conjugate coating by 1.6-3.7-fold. The release of anti-thrombotic prostaglandin I from endothelial cells was stimulated after nNitrite-nGH-collagen conjugate coating by 1.7-2.2-fold compared with collagen coating. Our data shows improved endothelialization and blood compatibility using nNitrite-nGH-collagen conjugate coating on silicone tubes suggesting that these coatings are highly suitable for use in blood-contacting parts of biomedical devices.
通过使与血液接触的部件内皮化以模拟血管内皮的功能，可以提高生物医学设备的生物相容性。使用仿生涂层可以实现更好的内皮化，这种涂层能够使生物活性分子（如抗血栓和促生长剂）从纳米脂质体中局部持续释放。我们旨在测试将促生长纳米脂质体生长激素（nGH）和抗血栓纳米脂质体亚硝酸钠（nNitrite）掺入硅胶管的胶原蛋白涂层中，是否能通过刺激内皮细胞增殖和抑制血小板黏附来增强内皮化。稳定固定在丙烯酸接枝硅胶管上的胶原蛋白涂层使水接触角从102°降至56°。将50或500nmol/ml的nNitrite和100或1000ng/ml的nGH掺入胶原蛋白涂层中，可使水接触角进一步降至48°。孵育120小时后，纳米脂质体中亚硝酸钠和生长激素初始量的58%的亚硝酸盐和22%的生长激素从nNitrite - nGH - 胶原蛋白涂层中逐渐释放。硅胶管用胶原蛋白表面涂层后，2天后内皮细胞数量增加了1.6倍，用nNitrite - nGH - 胶原蛋白共轭物涂层后增加了1.8 - 3.9倍。6天后，无表面涂层时内皮细胞汇合度为22%，胶原蛋白涂层时为74%，nNitrite - nGH - 胶原蛋白共轭物涂层时为83 - 119%。在没有内皮细胞的情况下，胶原蛋白涂层后血小板黏附增加了1.3倍，但nNitrite - nGH - 胶原蛋白共轭物涂层后血小板黏附受到抑制，降低了1.6 - 3.7倍。与胶原蛋白涂层相比，nNitrite - nGH - 胶原蛋白共轭物涂层后内皮细胞抗血栓前列腺素I的释放增加了1.7 - 2.2倍。我们的数据表明，在硅胶管上使用nNitrite - nGH - 胶原蛋白共轭物涂层可改善内皮化和血液相容性，这表明这些涂层非常适合用于生物医学设备的与血液接触部件。
