Palakollu Venkata Narayana, Thapliyal Neeta, Chiwunze Tirivashe E, Karpoormath Rajshekhar, Karunanidhi Sivanandhan, Cherukupalli Srinivasulu
Department of Pharmaceutical Chemistry, College of Health Sciences, University of KwaZulu-Natal (Westville Campus), Durban-4000, South Africa.
Department of Pharmaceutical Chemistry, College of Health Sciences, University of KwaZulu-Natal (Westville Campus), Durban-4000, South Africa.
Mater Sci Eng C Mater Biol Appl. 2017 Aug 1;77:394-404. doi: 10.1016/j.msec.2017.03.173. Epub 2017 Mar 22.
A facile preparation strategy based on electrochemical technique for the fabrication of glycine (Poly-Gly) and electrochemically reduced graphene oxide (ERGO) composite modified electrode was developed. The morphology of the developed composite (ERGO/Poly-Gly) was investigated using field emission scanning electron microscope (FE-SEM). The composite modified glassy carbon electrode (GCE) was characterized using fourier transform-infrared (FT-IR) spectroscopy, electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The electrochemical characterization results revealed that ERGO/Poly-Gly modified GCE has excellent electrocatalytic activity. Further, it was employed for sensing of l-dopa in pH5.5. Differential pulse voltammetry (DPV) was used for the quantification of l-dopa as well as for the simultaneous resolution of l-dopa and uric acid (UA). The LOD (S/N=3) was found to be 0.15μM at the proposed composite modified electrode. Determination of l-dopa could also be achieved in the presence of potentially interfering substances. The sensor showed high sensitivity and selectivity with appreciable reliability and precision. The proposed sensor was also successfully applied for real sample analysis.
开发了一种基于电化学技术的简便制备策略，用于制备甘氨酸（聚甘氨酸）和电化学还原氧化石墨烯（ERGO）复合修饰电极。使用场发射扫描电子显微镜（FE-SEM）研究了所制备复合材料（ERGO/聚甘氨酸）的形貌。使用傅里叶变换红外（FT-IR）光谱、电化学阻抗谱（EIS）和循环伏安法（CV）对复合修饰玻碳电极（GCE）进行了表征。电化学表征结果表明，ERGO/聚甘氨酸修饰的GCE具有优异的电催化活性。此外，它被用于在pH5.5条件下检测左旋多巴。差分脉冲伏安法（DPV）用于定量左旋多巴以及同时分辨左旋多巴和尿酸（UA）。在所提出的复合修饰电极上，检测限（S/N=3）为0.15μM。在存在潜在干扰物质的情况下也可以实现左旋多巴的测定。该传感器具有高灵敏度和选择性，具有可观的可靠性和精密度。所提出的传感器也成功应用于实际样品分析。
