Shi Yingge, Jiang Ruming, Liu Meiying, Fu Lihua, Zeng Guangjian, Wan Qing, Mao Liucheng, Deng Fengjie, Zhang Xiaoyong, Wei Yen
Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, PR China; School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, PR China.
Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, PR China.
Mater Sci Eng C Mater Biol Appl. 2017 Aug 1;77:972-977. doi: 10.1016/j.msec.2017.04.033. Epub 2017 Apr 7.
Polymeric fluorescent organic nanoparticles (polymer-FONs) have raised considerable research attention for biomedical applications owing to their advantages as compared with fluorescent inorganic nanoparticles and small organic molecules. In this study, we presented an efficient, facile and environment-friendly strategy to produce polymer-FONs, which relied on the self-polymerization of dopamine and polyethyleneimine (PEI) in rather mild conditions. To obtain the final polymer-FONs, aldehyde group-containing copolymers (named as poly(UA-co-PEGMA)) were synthesized by reversible addition-fragmentation chain-transfer polymerization using polyethylene glycol methyl ether methacrylate (PEGMA) and 1-undecen-10-al (UA) as monomers. The dopamine was conjugated onto poly(UA-co-PEGMA) through a multicomponent reaction between UA and dopamine to obtain poly(UA-co-PEGMA)-DA, which was further utilized for preparation of polymer-FONs through self-polymerization of dopamine and PEI. H nuclear magnetic resonance, Fourier transform infrared spectroscopy, transmission electron microscopy and fluorescence spectroscopy were employed to characterize the structure, morphology, compositions and optical properties of these polymer-FONs. Cell viability and cell uptake behavior results suggested that these polymer-FONs possess good biocompatibility and can be potentially utilized for biomedical applications. More importantly, the method can be also applied to fabricate many other multifunctional polymer-FONs with great potential for biomedical applications.
聚合物荧光有机纳米粒子（polymer-FONs）因其与荧光无机纳米粒子和有机小分子相比具有的优势，在生物医学应用方面引起了广泛的研究关注。在本研究中，我们提出了一种高效、简便且环境友好的策略来制备polymer-FONs，该策略依赖于多巴胺和聚乙烯亚胺（PEI）在相当温和的条件下进行自聚合。为了获得最终的polymer-FONs，以聚乙二醇甲基丙烯酸甲酯（PEGMA）和1-十一碳烯-10-醛（UA）作为单体，通过可逆加成-断裂链转移聚合反应合成了含醛基的共聚物（命名为聚（UA-co-PEGMA））。通过UA与多巴胺之间的多组分反应将多巴胺连接到聚（UA-co-PEGMA）上，得到聚（UA-co-PEGMA）-DA，其进一步通过多巴胺和PEI的自聚合用于制备polymer-FONs。采用氢核磁共振、傅里叶变换红外光谱、透射电子显微镜和荧光光谱对这些polymer-FONs的结构、形态、组成和光学性质进行了表征。细胞活力和细胞摄取行为结果表明，这些polymer-FONs具有良好的生物相容性，可潜在地用于生物医学应用。更重要的是，该方法还可应用于制备许多其他具有生物医学应用潜力的多功能polymer-FONs。
