Komatsu H, Tozawa H
Department of Immunology, School of Hygienic Sciences, Kitasato University, Kanagawa.
Microbiol Immunol. 1988;32(12):1201-10. doi: 10.1111/j.1348-0421.1988.tb01484.x.
An early stage of virus adsorption was studied in a system of Sendai virus metabolically labeled with [3H]leucine in LLCMK2 cells and of human red blood cells (RBCs). The efficiency of viral release from the virus-bound RBCs by incubation at 37 C depended on the number of virus particles which had been used for adsorption onto the RBC at 4 C. When 7.8 x 10(2) virus particles were previously adsorbed onto the RBC at 4 C, most of the viruses were dissociated from the RBC at 37 C. In the case of adsorption of 3 to 12 virus particles per RBC, however, most of the viruses were not dissociated from the RBC by incubation at 37 C. Such RBC-bound viruses were released by incubation with various bacterial neuraminidases (Clostridium perfringens, etc.) or with a large number of LLCMK2 cell-grown Sendai virus (LLCMK2-Sendai) particles, but not released by treatment with hemagglutinin-neuraminidase protein (Sendai-gp) isolated from egg-grown Sendai virus.
在一个用[3H]亮氨酸代谢标记仙台病毒的LLCMK2细胞与人红细胞（RBC）的系统中，研究了病毒吸附的早期阶段。通过在37℃孵育从结合病毒的红细胞中释放病毒的效率，取决于4℃时用于吸附到红细胞上的病毒颗粒数量。当7.8×10(2)个病毒颗粒预先在4℃吸附到红细胞上时，大多数病毒在37℃时从红细胞上解离。然而，在每个红细胞吸附3至12个病毒颗粒的情况下，大多数病毒在37℃孵育时不会从红细胞上解离。这种结合在红细胞上的病毒可通过与各种细菌神经氨酸酶（产气荚膜梭菌等）或大量在LLCMK2细胞中生长的仙台病毒（LLCMK2 - 仙台）颗粒孵育而释放，但不能通过用从鸡胚生长的仙台病毒中分离的血凝素 - 神经氨酸酶蛋白（仙台 - gp）处理而释放。
