Martin H, Loos M
Institute für Medizinische Mikrobiologie, Augustusplatz/Hochhaus, Mainz, F.R.G.
Mol Immunol. 1988 Dec;25(12):1231-7. doi: 10.1016/0161-5890(88)90037-5.
Biosynthetically labelled C1q secreted by guinea pig peritoneal macrophages was analysed by sedimentation through sucrose gradients followed by SDS-PAGE. In addition to the haemolytically active C1q of mol. wt 460,000 Da a low mol. wt (LMW) form of C1q was identified which had no detectable affinity for Fc of aggregated immunoglobulin, but which retained the ability to associate with the C1r2s2-complex. This LMW-C1q was covalently associated with two additional polypeptides of mol. wt 46 and 50 kDa.
通过蔗糖梯度沉降继以SDS-PAGE分析了豚鼠腹膜巨噬细胞分泌的生物合成标记的C1q。除了分子量为460,000 Da的具有溶血活性的C1q外，还鉴定出一种低分子量（LMW）形式的C1q，它对聚集免疫球蛋白的Fc没有可检测到的亲和力，但保留了与C1r2s2复合物结合的能力。这种LMW-C1q与另外两种分子量分别为46 kDa和50 kDa的多肽共价结合。
