Microbial Processes and Technology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695 019, India; Rajiv Gandhi Centre For Biotechnology, Trivandrum 695 014, India.
Microbial Processes and Technology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695 019, India.
Bioresour Technol. 2017 Dec;245(Pt B):1304-1313. doi: 10.1016/j.biortech.2017.05.031. Epub 2017 May 8.
Biocatalysts are creating increased interest among researchers due to their unique properties. Several enzymes are efficiently produced by microorganisms. However, the use of natural enzymes as biocatalysts is hindered by low catalytic efficiency and stability during various industrial processes. Many advanced enzyme technologies have been developed to reshape the existing natural enzymes to reduce these limitations and prospecting of novel enzymes. Frequently used enzyme technologies include protein engineering by directed evolution, immobilisation techniques, metagenomics etc. This review summarizes recent and emerging advancements in the area of enzyme technologies for the development of novel biocatalysts and further discusses the future directions in this field.
生物催化剂因其独特的性质在研究人员中引起了越来越多的兴趣。几种酶可以通过微生物高效生产。然而，由于天然酶在各种工业过程中的催化效率和稳定性较低，其作为生物催化剂的应用受到了限制。为了减少这些限制并探索新型酶，已经开发了许多先进的酶技术来重塑现有的天然酶。常用的酶技术包括定向进化的蛋白质工程、固定化技术、宏基因组学等。本文综述了酶技术在新型生物催化剂开发方面的最新进展，并进一步讨论了该领域的未来发展方向。
