来自胃肠线虫毛首鞭形线虫的排泄/分泌产物。

Excretory/secretory products from the gastrointestinal nematode Trichuris muris.

作者信息

Tritten Lucienne, Tam Mifong, Vargas Mireille, Jardim Armando, Stevenson Mary M, Keiser Jennifer, Geary Timothy G

机构信息

Institute of Parasitology, McGill University, 21,111 Lakeshore Road, Sainte-Anne-de-Bellevue, Quebec H9X 3V9, Canada; Centre for Host-Parasite Interactions, Canada.

Centre for Host-Parasite Interactions, Canada; Department of Microbiology and Immunology, McGill University, Montreal, Quebec H3A 2B4, Canada.

出版信息

Exp Parasitol. 2017 Jul;178:30-36. doi: 10.1016/j.exppara.2017.05.003. Epub 2017 May 19.

DOI:10.1016/j.exppara.2017.05.003

PMID:28533110

Abstract

To better control gastrointestinal nematode infections in humans and animals, it is important to understand the strategies used by these parasites to modulate the host immune system. In this regard, molecules released by parasites have been attributed crucially important roles in host-parasite negotiations. We characterized the excretory/secretory (E/S) microRNA (miRNA) and protein profiles from the mouse gastrointestinal nematode parasite Trichuris muris. Released miRNAs were subjected to miRNA sequencing and E/S proteins were analysed by mass spectrometry. Fourteen miRNAs were identified in T. muris exosome-like vesicles, as well as 73 proteins of nematode origin, 11 of which were unique to this study. Comparison with published nematode protein secretomes revealed high conservation at the functional level.

摘要

为了更好地控制人和动物的胃肠道线虫感染，了解这些寄生虫用于调节宿主免疫系统的策略非常重要。在这方面，寄生虫释放的分子在宿主与寄生虫的相互作用中发挥了至关重要的作用。我们对小鼠胃肠道线虫寄生虫毛首鞭形线虫的排泄/分泌（E/S）微小RNA（miRNA）和蛋白质谱进行了表征。对释放的miRNA进行了miRNA测序，并通过质谱分析了E/S蛋白。在毛首鞭形线虫的外泌体样囊泡中鉴定出14种miRNA，以及73种线虫来源的蛋白质，其中11种是本研究特有的。与已发表的线虫蛋白质分泌组比较显示，在功能水平上具有高度保守性。