Marvaud Jean-Christophe, Lambert Thierry
EA 4043, Unité Bactéries Pathogènes et Santé (UBaPS), Université Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France
EA 4043, Unité Bactéries Pathogènes et Santé (UBaPS), Université Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France.
Antimicrob Agents Chemother. 2017 Jul 25;61(8). doi: 10.1128/AAC.00301-17. Print 2017 Aug.
, which belongs to the complex, is a member of the human gut microbiota. Recent analysis of seven genomes of revealed the presence of an -like gene. Among these strains, only 90A7 was found to be resistant to rifampin in the absence of alteration of RpoB. Cloning of from 90A7 in combined with directed mutagenesis demonstrated that Arr-cb was functional but that a Q127→R variant present in 90A9 and 90B3 was inactive. Quantitative reverse transcription-PCR analysis indicated that was silent in the four remaining strains because of defective transcription. Thus, two independent mechanisms can make the probably intrinsic gene of cryptic.
属于该复合体的[具体名称未给出]是人类肠道微生物群的一员。最近对[具体名称未给出]的七个基因组的分析揭示了一个类似[具体名称未给出]的基因的存在。在这些菌株中，仅发现90A7在RpoB未发生改变的情况下对利福平耐药。将90A7中的[具体名称未给出]克隆到[具体名称未给出]中并结合定向诱变表明，Arr-cb具有功能，但90A9和90B3中存在的Q127→R变体无活性。定量逆转录PCR分析表明，由于转录缺陷，其余四个菌株中的[具体名称未给出]处于沉默状态。因此，两种独立的机制可使[具体名称未给出]可能的内在[具体名称未给出]基因变为隐性。
