Wang Jiangshan, Zhang Quan, Cui Feng, Hou Lei, Zhao Shuzhen, Xia Han, Qiu Jingjing, Li Tingting, Zhang Ye, Wang Xingjun, Zhao Chuanzhi
Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and PhysiologyJinan, China.
Yantai Institute of China Agricultural UniversityYantai, China.
Front Plant Sci. 2017 May 8;8:713. doi: 10.3389/fpls.2017.00713. eCollection 2017.
Low temperature is one of the major environmental stresses that affects plant growth and development, and leads to decrease in crop yield and quality. (salt cress) exhibits high tolerance to chilling, is an appropriate model to investigate the molecular mechanisms of cold tolerance. Here, we compared transcription changes in the roots and leaves of under cold stress using RNA-seq. We identified 2,782 and 1,430 differentially expressed genes (DEGs) in leaves and roots upon cold treatment, respectively. The expression levels of some genes were validated by quantitative real-time-PCR (qRT-PCR). Among these DEGs, 159 (11.1%) genes in roots and 232 (8.3%) genes in leaves were annotated as various types of transcription factors. We found that five aquaporin genes (three , one , and one responded to cold treatment. In addition, the expression of genes of -dependent cold signaling pathway genes altered in response to low temperature. KEGG pathway analysis indicated that these cold regulated genes were enriched in metabolism, photosynthesis, circadian rhythm, and transcriptional regulation. Our findings provided a complete picture of the regulatory network of cold stress response in . These cold-responsive genes could be targeted for detail functional study and utilization in crop cold tolerance improvement.
低温是影响植物生长发育的主要环境胁迫之一，会导致作物产量和品质下降。（盐芥）对低温具有高度耐受性，是研究耐寒分子机制的合适模型。在此，我们使用RNA测序比较了低温胁迫下盐芥根和叶中的转录变化。我们分别在冷处理后的叶和根中鉴定出2782个和1430个差异表达基因（DEG）。一些基因的表达水平通过定量实时PCR（qRT-PCR）进行了验证。在这些差异表达基因中，根中有159个（11.1%）基因和叶中有232个（8.3%）基因被注释为各种类型的转录因子。我们发现五个水通道蛋白基因（三个、一个和一个）对冷处理有反应。此外，依赖于的冷信号通路基因的基因表达响应低温而改变。KEGG通路分析表明，这些冷调控基因在代谢、光合作用、昼夜节律和转录调控中富集。我们的研究结果提供了盐芥冷胁迫响应调控网络的完整图景。这些冷响应基因可作为详细功能研究的靶点，并用于提高作物的耐寒性。
