Teuscher Matthias, Ströhlein Nadi, Birkenbach Markus, Schultheis Dorothea, Schoppmeier Michael
Department Biology, Developmental Biology Unit, Friedrich-Alexander-University Erlangen-Nuremberg, Staudtstr. 5, 91058 Erlangen, Germany.
Present address: Institute of Neuropathology, University Hospital Erlangen, Schwabachanlage 6, 91054 Erlangen, Germany.
Front Zool. 2017 May 19;14:26. doi: 10.1186/s12983-017-0212-2. eCollection 2017.
Stem cells are undifferentiated cells with a potential for self-renewal, which are essential to support normal development and homeostasis. To gain insight into the molecular mechanisms underlying adult stem cell biology and organ evolution, we use the telotrophic ovary of the beetle To this end, we participated in a large-scale RNAi screen in the red flour beetle , which identified functions in embryonic and postembryonic development for more than half of the genes.
We identified as candidate gene for the follicle stem cell linage in telotrophic oogenesis. belongs to the Casein Kinase 2 substrate family (CK2S), which in humans is associated with the proliferative activity of different cancers. Upon RNAi, central pre-follicular cells are lost, which results in termination of oogenesis. Given that also Notch-signalling is required to promote the mitotic activity of central pre-follicular cells, we performed epistasis experiments with and . In addition, we identified a putative follicle stem cell population by monitoring the mitotic pattern of wild type and depleted follicle cells by EdU incorporations. In RNAi these putative FSCs cease the expression of differentiation makers and are eventually lost.
depleted pre-follicular cells neither react to mitosis or endocycle stimulating signals, suggesting that provides competence for differentiation cues. This may resemble the situation in were CK2 is required to maintain the balance between proliferation and differentiation in the germ line. Since the earliest effect of RNAi is characterized by the loss of putative FSCs, we posit that crucially contributes to the proliferation or maintenance of follicle stem cells in the telotrophic ovary.
干细胞是具有自我更新潜能的未分化细胞，对支持正常发育和体内平衡至关重要。为深入了解成体干细胞生物学和器官进化的分子机制，我们利用甲虫的端滋养型卵巢。为此，我们参与了赤拟谷盗的大规模RNA干扰筛选，该筛选确定了超过一半的基因在胚胎发育和胚后发育中的功能。
我们确定 为端滋养型卵子发生中卵泡干细胞谱系的候选基因。 属于酪蛋白激酶2底物家族（CK2S），在人类中，该家族与不同癌症的增殖活性相关。RNA干扰后，卵泡前体细胞中央丢失，导致卵子发生终止。鉴于Notch信号传导也需要促进卵泡前体细胞中央的有丝分裂活性，我们用 和 进行了上位性实验。此外，我们通过EdU掺入监测野生型和 缺失卵泡细胞的有丝分裂模式，确定了一个假定的卵泡干细胞群体。在RNA干扰的 中，这些假定的卵泡干细胞停止表达分化标志物并最终丢失。
缺失的卵泡前体细胞对有丝分裂或内复制刺激信号均无反应，表明 为分化信号提供了能力。这可能类似于 在生殖系中需要CK2来维持增殖和分化之间平衡的情况。由于RNA干扰 的最早效应以假定的卵泡干细胞丢失为特征，我们认为 在端滋养型卵巢中对卵泡干细胞的增殖或维持起着关键作用。
