Dönitz Jürgen, Schmitt-Engel Christian, Grossmann Daniela, Gerischer Lizzy, Tech Maike, Schoppmeier Michael, Klingler Martin, Bucher Gregor
Johann-Friedrich-Blumenbach Institute of Zoology and Anthropology, GZMB, Department of Evolutionary Developmental Genetics, Georg-August-University Göttingen, 37075 Göttingen, Germany
Johann-Friedrich-Blumenbach Institute of Zoology and Anthropology, GZMB, Department of Evolutionary Developmental Genetics, Georg-August-University Göttingen, 37075 Göttingen, Germany Department of Biology, Friedrich-Alexander University, 91058 Erlangen, Germany.
Nucleic Acids Res. 2015 Jan;43(Database issue):D720-5. doi: 10.1093/nar/gku1054. Epub 2014 Nov 5.
The iBeetle-Base (http://ibeetle-base.uni-goettingen.de) makes available annotations of RNAi phenotypes, which were gathered in a large scale RNAi screen in the red flour beetle Tribolium castaneum (iBeetle screen). In addition, it provides access to sequence information and links for all Tribolium castaneum genes. The iBeetle-Base contains the annotations of phenotypes of several thousands of genes knocked down during embryonic and metamorphic epidermis and muscle development in addition to phenotypes linked to oogenesis and stink gland biology. The phenotypes are described according to the EQM (entity, quality, modifier) system using controlled vocabularies and the Tribolium morphological ontology (TrOn). Furthermore, images linked to the respective annotations are provided. The data are searchable either for specific phenotypes using a complex 'search for morphological defects' or a 'quick search' for gene names and IDs. The red flour beetle Tribolium castaneum has become an important model system for insect functional genetics and is a representative of the most species rich taxon, the Coleoptera, which comprise several devastating pests. It is used for studying insect typical development, the evolution of development and for research on metabolism and pest control. Besides Drosophila, Tribolium is the first insect model organism where large scale unbiased screens have been performed.
iBeetle数据库（http://ibeetle-base.uni-goettingen.de）提供了RNAi表型注释，这些注释是在赤拟谷盗（Tribolium castaneum）的大规模RNAi筛选（iBeetle筛选）中收集的。此外，它还提供了所有赤拟谷盗基因的序列信息和链接。iBeetle数据库除了包含与卵子发生和臭腺生物学相关的表型外，还包含了数千个在胚胎和变态表皮及肌肉发育过程中被敲低的基因的表型注释。这些表型根据EQM（实体、性质、修饰词）系统，使用受控词汇表和赤拟谷盗形态本体（TrOn）进行描述。此外，还提供了与各个注释相关的图像。数据既可以使用复杂的“搜索形态缺陷”功能按特定表型进行搜索，也可以通过“快速搜索”功能按基因名称和ID进行搜索。赤拟谷盗已成为昆虫功能遗传学的重要模型系统，是种类最丰富的分类单元鞘翅目中的代表，鞘翅目包含几种极具破坏力的害虫。它被用于研究昆虫典型发育、发育进化以及新陈代谢和害虫防治。除了果蝇，赤拟谷盗是第一个进行大规模无偏筛选的昆虫模式生物。
