Jobling M G, Peters S E, Ritchie D A
Department of Genetics and Microbiology, University of Liverpool, United Kingdom.
Plasmid. 1988 Sep;20(2):106-12. doi: 10.1016/0147-619x(88)90013-3.
The structural and functional properties of mercury resistance determinants cloned from a series of independently isolated conjugative plasmids were compared with those of the prototype HgR determinants from Tn501 and plasmid R100 (containing Tn21). Restriction endonuclease mapping classified the HgR determinants into at least three different but related structural groups which are distantly related to those from Tn501 and R100. These relationships were confirmed by the functional analysis of sub-clones and gamma delta insertion mutations and from the polypeptides specified by the cloned HgR determinants. Each mercury resistance clone synthesized polypeptides equivalent in size to the merA, merT, and merP gene products. However, those for merA and merT showed considerable size variation. No polypeptide equivalent to merD or merC of R100 was detected.
将从一系列独立分离的接合质粒中克隆出的汞抗性决定簇的结构和功能特性，与来自Tn501和质粒R100（含Tn21）的原型汞抗性决定簇的结构和功能特性进行了比较。限制性内切酶图谱分析将汞抗性决定簇分为至少三个不同但相关的结构组，这些结构组与来自Tn501和R100的结构组关系较远。通过亚克隆和γδ插入突变的功能分析以及克隆的汞抗性决定簇所指定的多肽，证实了这些关系。每个汞抗性克隆合成的多肽大小与merA、merT和merP基因产物相当。然而，merA和merT的多肽大小有相当大的差异。未检测到与R100的merD或merC相当的多肽。
