源自质粒R100的汞离子抗性基因中的Tn5插入突变。
Tn5 insertion mutations in the mercuric ion resistance genes derived from plasmid R100.
作者信息
Ni'Bhriain N N, Silver S, Foster T J
出版信息
J Bacteriol. 1983 Aug;155(2):690-703. doi: 10.1128/jb.155.2.690-703.1983.
Abstract
The mercuric resistance (mer) genes of plasmid R100 were cloned into plasmid pBR322. A series of transposon Tn5 insertion mutations in the mer genes were isolated and mapped. The mutants were characterized phenotypically by their sensitivity to Hg2+ and by binding and volatilization of 203Hg2+. Dominance and complementation tests were also performed. Mutations affecting the previously described mer genes merR (regulation), merT (transport), and merA (reductase) were characterized. Evidence was obtained for two new mer genes, which have been called merC and merD. A restriction enzyme map of the mer region was drawn with the gene order merRTCAD. Transcriptional merR-lac and merA-lac fusions were generated by insertion of phage Mu d amp lac into plasmid R100-1. These were used to study regulation of mer gene expression. The merR gene product appears to regulate negatively its own expression as well as acting as both a negative and a positive regulator of the merTCA genes.
摘要
质粒R100的汞抗性（mer）基因被克隆到质粒pBR322中。分离并定位了mer基因中一系列转座子Tn5插入突变。通过它们对Hg2+的敏感性以及203Hg2+的结合和挥发对突变体进行表型鉴定。还进行了显性和互补测试。对影响先前描述的mer基因merR（调控）、merT（转运）和merA（还原酶）的突变进行了表征。获得了两个新的mer基因的证据，它们被称为merC和merD。绘制了mer区域的限制性酶切图谱，基因顺序为merRTCAD。通过将噬菌体Mu d amp lac插入质粒R100-1中产生转录merR-lac和merA-lac融合体。这些用于研究mer基因表达的调控。merR基因产物似乎对其自身表达进行负调控，同时作为merTCA基因的负调控和正调控因子发挥作用。
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