Nielsen Shaun, Wilkes Walburn Jackson, Vergés Adriana, Thomas Torsten, Egan Suhelen
Centre for Marine Bio-Innovation and School of Biological Earth and Environmental Sciences, University of New South Wales, Sydney, Australia.
PeerJ. 2017 May 17;5:e3317. doi: 10.7717/peerj.3317. eCollection 2017.
Most of our knowledge regarding the biodiversity of gut microbes comes from terrestrial organisms or marine species of economic value, with less emphasis on ecologically important species. Here we investigate the bacterial composition associated with the gut of , a rabbitfish that plays an important ecological role in coastal ecosystems by consuming seaweeds. Members of Firmicutes, Bacteroidetes and delta-Proteobacteria were among the dominant taxa across samples taken from the contents and the walls (sites) of the midgut and hindgut (location). Despite the high variability among individual fish, we observed statistically significant differences in beta-diversity between gut sites and gut locations. Some bacterial taxa low in abundance in the midgut content (e.g., ) were found in greater abundances on the midgut wall and within the hindgut, suggesting that the gut may select for specific groups of environmental and/or food-associated microorganisms. In contrast, some distinct taxa present in the midgut content (e.g., ) were noticeably reduced in the midgut wall and hindgut, and are thus likely to be representative of transient microbiota. This is the first assessment of the bacterial diversity associated with the gut of and highlights the need to consider the variability across different gut locations and sites when analyzing fish gut microbiomes.
我们关于肠道微生物多样性的大部分知识来自陆地生物或具有经济价值的海洋物种，而对具有重要生态意义的物种关注较少。在此，我们研究了与一种以食用海藻在沿海生态系统中发挥重要生态作用的蓝子鱼肠道相关的细菌组成。厚壁菌门、拟杆菌门和δ-变形菌门的成员是从中肠和后肠内容物及肠壁（部位）采集的样本中的优势类群。尽管个体鱼之间存在高度变异性，但我们观察到肠道部位和肠道位置之间的β-多样性存在统计学上的显著差异。一些在中肠内容物中丰度较低的细菌类群（如 ）在中肠壁和后肠中丰度更高，这表明肠道可能会选择特定的环境和/或与食物相关的微生物群。相反，一些存在于中肠内容物中的独特类群（如 ）在中肠壁和后肠中明显减少，因此可能代表短暂的微生物群。这是对与蓝子鱼肠道相关的细菌多样性的首次评估，并强调在分析鱼类肠道微生物群时需要考虑不同肠道位置和部位的变异性。
