Parameswari R, Rao K A, Manigandan P, Vickram A S, Archana A, Sridharan T B
Department of Industrial Biotechnology, School of Biosciences and Technology, VIT University, Vellore, India.
Cryo Letters. 2017 Mar/Apr;38(2):95-99.
Antioxidants protect spermatozoa against lipid peroxidation during freezing.
The study is designed to elucidate the suitable extender to preserve infertile semen of smokers against ROS damage using natural Tea polyphenol (T. arjuna bark extract).
Forty-two infertile subjects with smoking habit and 28 fertile subjects without smoking habit were considered for the study. Four semen extenders including our naturally derived antioxidant component were prepared and used to preserve semen sample from the study subjects for a period of one month. Standard semen parameters, biochemical and sperm DNA damage marker with inhibition were measured before and after cryopreservation.
The motility and morphology of sperm cells were maintained better in E4 extender, and DNA damage is reduced.
Extender recipe with natural antioxidants (E3 and E4) was found to be apt for infertile semen preservation.
抗氧化剂可保护精子在冷冻过程中免受脂质过氧化损伤。
本研究旨在阐明使用天然茶多酚（阿朱那树皮提取物）保存吸烟者不育精液免受活性氧损伤的合适稀释液。
本研究纳入42名有吸烟习惯的不育受试者和28名无吸烟习惯的生育受试者。制备了四种精液稀释液，包括我们天然衍生的抗氧化成分，并用于保存研究对象的精液样本一个月。在冷冻保存前后测量标准精液参数、生化指标和精子DNA损伤标志物及抑制情况。
E4稀释液能更好地维持精子细胞的活力和形态，且DNA损伤减少。
发现含有天然抗氧化剂的稀释液配方（E3和E4）适用于不育精液的保存。
