Ranganathan Parameswari, Sridharan T B, Babujanarthanam R, Madhan Kumar P, Ganesamoorthy R, Thirugnanasambandham K
Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632115, India.
SMV 117, Gene Cloning and Technology lab, SBST, VIT, Vellore, India.
Toxicol Rep. 2024 Aug 20;13:101714. doi: 10.1016/j.toxrep.2024.101714. eCollection 2024 Dec.
Oil-mediated nano emulsion, a more advanced technology than other commercial extenders, will protect spermatozoa for extended periods against ROS and cryo injuries. The study is designed to elucidate the most suitable extender to preserve infertile subjects' sperm cells against ROS damage. This is a significant step forward for the andrology society, as it introduces the use of a nano emulsion extender (natural oil extract) for the first time, particularly for those who are undergoing urology-related defects. The study involved forty-four (44) infertile subjects with smoking habits and forty-two (42) fertile subjects without smoking habits, as per CASA guidelines. Semen preservatives (glycerol and egg yolk citrate), along with our naturally derived nano emulsion components (CN, EN, and EL), were prepared and used to preserve the semen sample for 24 hours. Standard semen parameters (morphology, viability, and integrity), ROS, and sperm acrosome state by staining were measured before and after cryopreservation. The results indicated that the morphology and motility of sperm features were better maintained in the CN-oil-mediated nano emulsion than in other emulsions, and ROS-induced DNA damage was reduced.
油介导的纳米乳液是一种比其他商业稀释剂更先进的技术，它能长时间保护精子免受活性氧（ROS）和冷冻损伤。该研究旨在阐明最适合保存不育男性精子细胞免受ROS损伤的稀释剂。这是男科学领域向前迈出的重要一步，因为它首次引入了纳米乳液稀释剂（天然油提取物）的使用，特别是对于那些患有与泌尿外科相关缺陷的人。根据计算机辅助精液分析（CASA）指南，该研究纳入了44名有吸烟习惯的不育男性和42名无吸烟习惯的生育男性。制备了精液防腐剂（甘油和蛋黄柠檬酸盐）以及我们天然衍生的纳米乳液成分（CN、EN和EL），并用于保存精液样本24小时。在冷冻保存前后，通过染色测量标准精液参数（形态、活力和完整性）、ROS以及精子顶体状态。结果表明，与其他乳液相比，CN油介导的纳米乳液能更好地维持精子的形态和活力，并且减少了ROS诱导的DNA损伤。
