Chen S M, Ren L, Zhang D, Zhang Y F, Shen X H
Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai, China.
Cryo Letters. 2017 Mar/Apr;38(2):125-136.
Adding exogenous compounds is an effective way to improve cell survival after cryopreservation. Carbon nanomaterials (CNMs) are novel exogenous substances with small particle size and good biocompatibility.
In this work, four types of CNMs were used for the cryopreservation of Agapanthus praecox callus and their possible effects and mechanism of action were analyzed.
The thermal properties of the vitrification solutions tested were detected by differential scanning calorimetry. Raman spectroscopy and transmission electron microscopy analyses were used to study the distribution of CNMs inside cells. The MDA/H2O2 contents were measured to evaluate the toxicity of CNMs to cells.
Supplementation of PVS2 with various CNMs at different concentrations could enhance survival. The most effective concentration was 0.3 g/L C60, which increased survival by 159% compared to untreated controls and decreased the MDA and H2O2 contents. Single-walled carbon nanotubes (SWCNTs) and C60 entered callus cells. C60 was found only in mitochondria, whereas SWCNTs were located only around the cell walls.
添加外源化合物是提高细胞冷冻保存后存活率的有效方法。碳纳米材料（CNMs）是一类新型的外源物质，具有粒径小、生物相容性好的特点。
本研究使用四种类型的碳纳米材料对百子莲愈伤组织进行冷冻保存，并分析其可能的作用效果及作用机制。
采用差示扫描量热法检测所测试玻璃化溶液的热性能。利用拉曼光谱和透射电子显微镜分析研究碳纳米材料在细胞内的分布情况。通过测量丙二醛/过氧化氢含量评估碳纳米材料对细胞的毒性。
在不同浓度下向PVS2中添加各种碳纳米材料均可提高存活率。最有效的浓度为0.3 g/L的C60，与未处理的对照组相比，其使存活率提高了159%，并降低了丙二醛和过氧化氢的含量。单壁碳纳米管（SWCNTs）和C60进入了愈伤组织细胞。发现C60仅存在于线粒体中，而SWCNTs仅位于细胞壁周围。
