Xu Miao, Song Yu, Zhang Jingjinqiu, Zhao Yeyu, Geng Huaman, Weng Xiaogang, Liu Zhonghua, Cheng Jinju, Yan Tingsheng
Key Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
School of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Jul 25;40(7):2294-2307. doi: 10.13345/j.cjb.230889.
Extensive studies have been conducted on deicing nanomaterials to improve the cryoprotective effects on cells, tissues, and organs. The nanomaterials with different composition, sizes, and shapes can inhibit the formation and growth of ice crystals, thereby reducing the damage to the cryopreserved samples. In this study, the carbon composite particles (CCPs) with different sizes and shapes were prepared by the hydrothermal method. The results demonstrated that the cryoprotective effect of CCPs enhanced with the decrease in particle size. Compared with spherical CCPs, Janus nanoparticles and WSP nanoflower with special shapes demonstrated improved protective effects on cryopreserved cells. In addition, the combination of deicing micro/nanomaterials at appropriate concentrations with commercial cryoprotectants exerted improved cryoprotective effects on cells. The prepared deicing micro/nanomaterials can improve cell cryopreservation, demonstrating great application potential in biomedical research and cryopreservation.
为提高对细胞、组织和器官的冷冻保护效果，人们对用于除冰的纳米材料进行了广泛研究。具有不同组成、尺寸和形状的纳米材料可以抑制冰晶的形成和生长，从而减少对冷冻保存样本的损伤。在本研究中，通过水热法制备了不同尺寸和形状的碳复合颗粒（CCP）。结果表明，CCP的冷冻保护效果随粒径减小而增强。与球形CCP相比，具有特殊形状的Janus纳米颗粒和WSP纳米花对冷冻保存的细胞表现出更好的保护效果。此外，将适当浓度的除冰微/纳米材料与商业冷冻保护剂组合使用，对细胞具有更好的冷冻保护效果。所制备的除冰微/纳米材料可以改善细胞冷冻保存，在生物医学研究和冷冻保存中显示出巨大的应用潜力。
