Steadman L E, Murdoch W J
Department of Animal Science, University of Wyoming, Laramie 82071.
Prostaglandins. 1988 Nov;36(5):741-5. doi: 10.1016/0090-6980(88)90017-2.
Sheep were injected on Day 10 of the estrous cycle with a luteolytic dose of prostaglandin (PG) F2 alpha or injection vehicle. Corpora lutea were recovered at laparatomy 2, 4, 8, and 16 h following treatment. Portions of tissue were processed for light microscopy and analyzed for concentrations of leukotriene (LT) B4 and progesterone. There was a significant increase in LTB4 in tissues collected before evidence of either functional or structural regression (2 h); such levels were sustained. Tissues isolated 2 h after administration of PGF2 alpha and placed in short-term incubation produced considerable quantities of LTB4. Although no direct in vitro effect of PGF2 alpha on luteal production of LTB4 was demonstrated, it seems possible that lipoxygenase products of arachidonate metabolism, such as LTB4, could act as intermediates in the luteolytic mechanism of action of PGF2 alpha in vivo.
在发情周期的第10天，给绵羊注射溶黄体剂量的前列腺素（PG）F2α或注射媒介物。在处理后2、4、8和16小时通过剖腹术回收黄体。将部分组织进行处理用于光学显微镜检查，并分析白三烯（LT）B4和孕酮的浓度。在出现功能或结构退化迹象之前（2小时）收集的组织中，LTB4显著增加；此类水平持续存在。在给予PGF2α后2小时分离的组织并进行短期培养，产生了大量的LTB4。尽管未证明PGF2α对黄体产生LTB4有直接的体外作用，但花生四烯酸代谢的脂氧合酶产物（如LTB4）似乎有可能在体内作为PGF2α溶黄体作用机制的中间体。
