Carvalho C B, Yeik B S, Murdoch W J
Dept. of Animal Science, University of Wyoming, Laramie 82071.
Prostaglandins. 1989 May;37(5):553-8. doi: 10.1016/0090-6980(89)90070-1.
Concurrent changes in concentrations of a product of the cyclooxygenase (prostaglandin [PG] F2 alpha) and lipoxygenase (leukotriene [LT] B4) routes of metabolism of arachidonic acid were measured by radioimmunoassay within the wall of periovulatory ovine follicles. Increased concentrations of PGF2 alpha were detected within follicles before, during and following the time of ovulation. A significant rise in LTB4 was not observed until after follicular rupture had occurred. Inhibition of synthesis of PGF2 alpha by indomethacin was associated with a complete blockade of ovulation. Nordihydroguaiaretic acid, an inhibitor of 5-lipoxygenase, had no effect on ovulation. Periovulatory administration of either drug did not alter sera profiles of progesterone during subsequent luteal phases. These results reconfirm the importance of the cyclooxygenase system in the mechanism of ovulation. It does not appear that follicular LTB4 is a key component in the processes of ovulation or luteinization in sheep.
通过放射免疫测定法，在排卵前绵羊卵泡壁内检测了花生四烯酸代谢的环氧化酶途径（前列腺素[PG]F2α）和脂氧合酶途径（白三烯[LT]B4）产物浓度的同时变化。在排卵前、排卵期间和排卵后，卵泡内均检测到PGF2α浓度升高。直到卵泡破裂后才观察到LTB4显著升高。吲哚美辛抑制PGF2α的合成与排卵的完全阻断有关。5-脂氧合酶抑制剂去甲二氢愈创木酸对排卵没有影响。在排卵前后给予这两种药物中的任何一种，在随后的黄体期均未改变血清孕酮水平。这些结果再次证实了环氧化酶系统在排卵机制中的重要性。卵泡LTB4似乎不是绵羊排卵或黄体化过程中的关键成分。
