Cetin Idil, Topcul Mehmet R
Istanbul University, Faculty of Science, Department of Biology, Istanbul, Turkey.
J BUON. 2017 Mar-Apr;22(2):347-354.
In this study, the in vitro cytotoxic effect of nanotechnological drugs nab-paclitaxel and liposomal cisplatin combination was evaluated on MDA-MB-231 and MCF-7 breast cancer cell lines.
For this purpose cell viability, cell index values obtained from xCELLigence RTCA (Real-Time Cell Analysis) DP instrument, mitotic index (MI), apoptotic index (AI) and labelling index (LI) analysis among cell kinetic parameters were used. A1L25: 1 μg/ml nab-paclitaxel+25 μg/ml liposomal cisplatin, A1L5: 1 μg/ml nab-paclitaxel+5 μg/ml liposomal cisplatin and A10L5: 10 μg/ml nab-paclitaxel+5 μg/ml liposomal cisplatin for MDA-MB-231 cell line and A1L5: 1 μg/ml nab-paclitaxel+5 μg/ml liposomal cisplatin, A1L10: 1 μg/ml nab-paclitaxel+10 μg/ml liposomal cisplatin and A5L1: 5 μg/ml nab-paclitaxel+1 μg/ml liposomal cisplatin doses for MCF-7 were applied for 24-72 hrs.
Significant decrease in cell viability and cell index values for both cell lines was observed, while the MI and LI values of both cell lines increased at 24 hrs, and decreased significantly at 72 hrs. Also there was a significant increase in the AI values.
Nab-paclitaxel and liposomal cisplatin offer a promising treatment modality in different breast cancer subtypes.
本研究评估了纳米技术药物纳米白蛋白结合型紫杉醇和脂质体顺铂联合用药对MDA-MB-231和MCF-7乳腺癌细胞系的体外细胞毒性作用。
为此，使用了细胞活力、从xCELLigence RTCA（实时细胞分析）DP仪器获得的细胞指数值、细胞动力学参数中的有丝分裂指数（MI）、凋亡指数（AI）和标记指数（LI）分析。对于MDA-MB-231细胞系，使用A1L25：1μg/ml纳米白蛋白结合型紫杉醇+25μg/ml脂质体顺铂、A1L5：1μg/ml纳米白蛋白结合型紫杉醇+5μg/ml脂质体顺铂和A10L5：10μg/ml纳米白蛋白结合型紫杉醇+5μg/ml脂质体顺铂；对于MCF-7细胞系，使用A1L5：1μg/ml纳米白蛋白结合型紫杉醇+5μg/ml脂质体顺铂、A1L10：1μg/ml纳米白蛋白结合型紫杉醇+10μg/ml脂质体顺铂和A5L1：5μg/ml纳米白蛋白结合型紫杉醇+1μg/ml脂质体顺铂的剂量，作用24至72小时。
观察到两种细胞系的细胞活力和细胞指数值均显著降低，而两种细胞系的MI和LI值在24小时时升高，在72小时时显著降低。此外，AI值也显著增加。
纳米白蛋白结合型紫杉醇和脂质体顺铂为不同乳腺癌亚型提供了一种有前景的治疗方式。
