Tsyplenkov P V, Morozov V I, Kokriakov V N, Rogozkin V A
Ukr Biokhim Zh (1978). 1988 Nov-Dec;60(6):72-5.
The immunoradiometric assay of rat myeloperoxidase (MPO) was developed based on the usage of the CNBr-activated sepharose as a solid phase for antiMPO and of [125I] IgG (the affinity preparation of antiMPO) as a label. The analysis lasted for 8 h and standard curve limits were 0.5-500 ng per tube. Intra and inter assay coefficients of variation did not exceed 3% and 6.5%, respectively. It was shown by the above method that the intensive swimming of male rats led to an increase in the MPO concentration in blood plasma and to its decrease in neutrophils.
大鼠髓过氧化物酶（MPO）的免疫放射分析是基于使用溴化氰活化的琼脂糖作为抗MPO的固相载体以及使用[125I]IgG（抗MPO的亲和制剂）作为标记物而开发的。分析持续8小时，标准曲线范围为每管0.5 - 500纳克。批内和批间变异系数分别不超过3%和6.5%。通过上述方法表明，雄性大鼠的高强度游泳导致血浆中MPO浓度升高，而中性粒细胞中MPO浓度降低。
