Ma Wenqiang, Lu Jingyu, Jiang Shuxia, Cai Demin, Pan Shifeng, Jia Yimin, Zhao Ruqian
1Key Laboratory of Animal Physiology and Biochemistry,College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,People's Republic of China.
Br J Nutr. 2017 Apr;117(7):923-929. doi: 10.1017/S0007114517000794.
To investigate the effects of maternal dietary protein restriction on offspring Fe metabolism, twenty-four second-parity Landrace×Yorkshire sows were randomly allocated to standard-protein (SP) and low-protein (LP) groups. The SP sows were fed diets containing 15 and 18 % crude protein throughout pregnancy and lactation, respectively, whereas the LP sows were subjected to 50 % dietary protein restriction. Offspring birth weight was not affected, but the body weight at weaning (P=0·06) and average daily gain (P=0·01) of the female piglets were significantly decreased. Serum Fe level in the LP piglets was markedly decreased at weaning, especially in males (P=0·03). Serum ferritin level (P=0·08) tended to be lower, yet serum transferrin was greatly higher (P=0·01) in male weaning piglets of the LP group. Duodenal expression of the divalent metal transporter 1 (DMT1) and ferroportin (FPN) was surprisingly reduced (P<0·05) at the level of protein, but not at the mRNA level, in male weaning piglets of the LP group. Male weaning piglets born to the LP sows exhibited higher hepatic hepcidin levels (P=0·09), lower hepatic expression of transferrin (P<0·01) and transferrin receptor 1 (P<0·05) at the level of mRNA. However, no significant differences were observed for hepatic Fe storage, ferritin, transferrin and transferrin receptor 1 protein expression in male weaning piglets of the two groups. These results indicate that maternal protein restriction during pregnancy and lactation influences growth of female offspring at weaning, reduces duodenal expression of Fe transporters (DMT1 and FPN) and decreases serum Fe level in male weaning piglets.
为研究母体膳食蛋白质限制对后代铁代谢的影响，将24头经产长白×约克夏母猪随机分为标准蛋白（SP）组和低蛋白（LP）组。SP组母猪在整个妊娠和哺乳期分别饲喂含15%和18%粗蛋白的日粮，而LP组母猪的日粮蛋白质限制为50%。后代出生体重未受影响，但雌性仔猪断奶时体重（P = 0.06）和平均日增重（P = 0.01）显著降低。LP组仔猪断奶时血清铁水平显著降低，尤其是雄性仔猪（P = 0.03）。LP组雄性断奶仔猪血清铁蛋白水平有降低趋势（P = 0.08），而血清转铁蛋白水平显著升高（P = 0.01）。LP组雄性断奶仔猪十二指肠中二价金属转运体1（DMT1）和铁转运蛋白（FPN）的蛋白表达水平意外降低（P < 0.05），但mRNA水平未降低。LP组母猪所生雄性断奶仔猪肝脏中肝素水平较高（P = 0.09），mRNA水平下肝脏中转铁蛋白（P < 0.01）和转铁蛋白受体1（P < 0.05）的表达较低。然而，两组雄性断奶仔猪肝脏铁储存、铁蛋白、转铁蛋白和转铁蛋白受体1蛋白表达未观察到显著差异。这些结果表明，妊娠和哺乳期母体蛋白质限制会影响雌性后代断奶时的生长，降低十二指肠中铁转运蛋白（DMT1和FPN）的表达，并降低雄性断奶仔猪的血清铁水平。
