Kim In Hye, Lee Tae-Kyeong, Cho Jeong Hwi, Lee Jae-Chul, Park Joon Ha, Ahn Ji Hyeon, Shin Bich-Na, Chen Bai Hui, Tae Hyun-Jin, Kim Yang Hee, Kim Jong-Dai, Kim Young-Myeong, Won Moo-Ho, Kang Il Jun
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea.
Mol Med Rep. 2017 Jul;16(1):133-142. doi: 10.3892/mmr.2017.6591. Epub 2017 May 17.
Chrysanthemum indicum Linné extract (CIL) is used in herbal medicine in East Asia. In the present study, gerbils were orally pre‑treated with CIL, and changes of antioxidant enzymes including superoxide dismutase (SOD) 1 and SOD2, catalase (CAT) and glutathione peroxidase (GPX) in the hippocampal CA1 region following 5 min of transient cerebral ischemia were investigated and the neuroprotective effect of CIL in the ischemic CA1 region was examined. SOD1, SOD2, CAT and GPX immunoreactivities were observed in the pyramidal cells of the CA1 region and their immunoreactivities were gradually decreased following ischemia‑reperfusion and barely detectable at 5 days post‑ischemia. CIL pre‑treatment significantly increased immunoreactivities of SOD1, CAT and GPX, but not SOD2, in the CA1 pyramidal cells of the sham‑operated animals. In addition, SOD1, SOD2, CAT and GPX immunoreactivities in the CA1 pyramidal cells were significantly higher compared with the ischemia‑operated animals. Furthermore, it was identified that pre‑treatment with CIL protected the CA1 pyramidal cells in the CA1 region using neuronal nuclei immunohistochemistry and Fluoro‑Jade B histofluorescence staining; the protected CA1 pyramidal cells were 67.5% compared with the sham‑operated animals. In conclusion, oral CIL pre‑treatment increased endogenous antioxidant enzymes in CA1 pyramidal cells in the gerbil hippocampus and protected the cells from transient cerebral ischemic insult. This finding suggested that CIL is promising for the prevention of ischemia‑induced neuronal damage.
野菊花提取物（CIL）在东亚被用于草药医学。在本研究中，对沙鼠进行CIL口服预处理，研究短暂性脑缺血5分钟后海马CA1区超氧化物歧化酶（SOD）1和SOD2、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）等抗氧化酶的变化，并检测CIL对缺血CA1区的神经保护作用。在CA1区锥体细胞中观察到SOD1、SOD2、CAT和GPX的免疫反应性，缺血再灌注后其免疫反应性逐渐降低，在缺血后5天几乎检测不到。CIL预处理显著增加了假手术动物CA1锥体细胞中SOD1、CAT和GPX的免疫反应性，但未增加SOD2的免疫反应性。此外，与缺血手术动物相比，CA1锥体细胞中SOD1、SOD2、CAT和GPX的免疫反应性显著更高。此外，通过神经元细胞核免疫组织化学和荧光金B组织荧光染色确定，CIL预处理可保护CA1区的CA1锥体细胞；与假手术动物相比，受保护的CA1锥体细胞为67.5%。总之，口服CIL预处理可增加沙鼠海马CA1锥体细胞内源性抗氧化酶，并保护细胞免受短暂性脑缺血损伤。这一发现表明CIL在预防缺血性神经元损伤方面具有前景。
