Yoo Ki-Yeon, Kim In Hye, Cho Jeong-Hwi, Ahn Ji Hyeon, Park Joon Ha, Lee Jae-Chul, Tae Hyun-Jin, Kim Dae Won, Kim Jong-Dai, Hong Seongkweon, Won Moo-Ho, Kang Il Jun
Department of Oral Anatomy, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University, Gangneung, Republic of Korea.
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea.
Neural Regen Res. 2016 Feb;11(2):270-7. doi: 10.4103/1673-5374.177735.
In this study, we tried to verify the neuroprotective effect of Chrysanthemum indicum Linne (CIL) extract, which has been used as a botanical drug in East Asia, against ischemic damage and to explore the underlying mechanism involving the anti-inflammatory approach. A gerbil was given CIL extract for 7 consecutive days followed by bilateral carotid artery occlusion to make a cerebral ischemia/reperfusion model. Then, we found that CIL extracts protected pyramidal neurons in the hippocampal CA1 region (CA1) from ischemic damage using neuronal nucleus immunohistochemistry and Fluoro-Jade B histofluorescence. Accordingly, interleukin-13 immunoreactivities in the CA1 pyramidal neurons of CIL-pretreated animals were maintained or increased after cerebral ischemia/reperfusion. These findings indicate that the pre-treatment of CIL can attenuate neuronal damage/death in the brain after cerebral ischemia/reperfusion via an anti-inflammatory approach.
在本研究中，我们试图验证野菊花提取物（CIL）对缺血损伤的神经保护作用，该提取物在东亚地区已被用作植物药，并探讨其涉及抗炎途径的潜在机制。将沙土鼠连续7天给予CIL提取物，随后进行双侧颈动脉闭塞以建立脑缺血/再灌注模型。然后，我们通过神经元细胞核免疫组织化学和荧光金B组织荧光法发现，CIL提取物可保护海马CA1区（CA1）的锥体神经元免受缺血损伤。因此，在脑缺血/再灌注后，CIL预处理动物的CA1锥体神经元中的白细胞介素-13免疫反应性保持或增加。这些发现表明，CIL预处理可通过抗炎途径减轻脑缺血/再灌注后大脑中的神经元损伤/死亡。
