Kim Tae-Won, Kim Young-Jung, Park So-Ra, Seo Chang-Seob, Ha Hyekyung, Shin Hyeun-Kyoo, Jung Ju-Young
Nat Prod Commun. 2015 Mar;10(3):397-402.
Chrysanthemum indicum Linné has been used in traditional medicine to treat various inflammatory diseases in East Asia. The aim of the present study was to investigate the protective effect of C. indicum ethanol extract (CILE) against cisplatin-induced nephrotoxicity. An HPLC-photodiode array method was used for fingerprint analysis of the CILE and ten major constituents were quantitatively analyzed. The protective effect of CILE on cisplatin-induced nephrotoxicity was assessed using both in vitro (porcine kidney cell; PK15 cell) and in vivo (Sprague Dawley rat) experiments. In the in vitro study, CILE enhanced PK15 cell viability after cisplatin treatment with recovered antioxidant status. Moreover, the increased p53 expression after cisplatin treatment was decreased in the CILE pretreated cells. In the in vivo study, SD rats were treated for 28 consecutive days with CILE (0, 100, 300 and 500 mg/kg). On day 23, a single dose of cisplatin (5 mg/kg) was injected to induce nephrotoxicity. The CILE pretreated group showed recovered serum renal function index with ameliorated oxidative stress. Histopathological alterations and apoptosis in the kidney were also decreased in CILE pretreated rats. Taken together, CILE could attenuate cisplatin-induced nephrotoxicity and might be a beneficial agent for acute renal failure management.
野菊花在东亚传统医学中一直被用于治疗各种炎症性疾病。本研究的目的是探讨野菊花乙醇提取物（CILE）对顺铂诱导的肾毒性的保护作用。采用高效液相色谱-光电二极管阵列法对CILE进行指纹图谱分析，并对十种主要成分进行定量分析。通过体外（猪肾细胞；PK15细胞）和体内（Sprague Dawley大鼠）实验评估CILE对顺铂诱导的肾毒性的保护作用。在体外研究中，CILE在用顺铂处理后提高了PK15细胞的活力，并恢复了抗氧化状态。此外，在CILE预处理的细胞中，顺铂处理后增加的p53表达降低。在体内研究中，SD大鼠连续28天接受CILE（0、100、300和500mg/kg）治疗。在第23天，注射单剂量顺铂（5mg/kg）以诱导肾毒性。CILE预处理组的血清肾功能指标恢复，氧化应激得到改善。CILE预处理的大鼠肾脏的组织病理学改变和细胞凋亡也减少。综上所述，CILE可以减轻顺铂诱导的肾毒性，可能是治疗急性肾衰竭的有益药物。
