Meng Fanming, Ren Lipin, Wang Ziyue, Deng Jianqiang, Guo Yadong, Chen Chao, Finkelbergs Dmitrijs, Cai Jifeng
Department of Histology and Embryology, School of Basic Medical Sciences, Central South University, Changsha 410078, Hunan, China.
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, Hunan, China.
J Med Entomol. 2017 Sep 1;54(5):1193-1200. doi: 10.1093/jme/tjx105.
Blow flies are among the most important insects in forensic entomology casework. Identification of blow fly species can be a time consuming and difficult task, especially at their early development stages. Present DNA-based technologies provide a promising identification method for these forensically important calliphorids. The cytochrome oxidase subunit I (COI) sequence has been applied as a suitable DNA marker in calliphorid identification for many years; however, limitation exists in using short sequence to determine genetically close species. In this study, COI long sequences were utilized in species-level identification. Seventy-two specimens were collected from 27 locations across 22 Chinese provinces, and unambiguously determined as 16 species under seven genera of Calliphoridae. Analysis of long mitochondrial COI sequence (1,021-1,382 bp) data from forensically relevant blow flies collected in the inland region of China provided a reliable marker for accurate identification. Our data provide genetic diversity and reference for global forensic-related blow fly species identification, and conductive meaning on future utilization of Chinese calliphorids used in forensic entomological practice.
丽蝇是法医昆虫学案件中最重要的昆虫之一。丽蝇物种的鉴定可能是一项耗时且困难的任务，尤其是在其早期发育阶段。目前基于DNA的技术为这些对法医重要的丽蝇科昆虫提供了一种有前景的鉴定方法。细胞色素氧化酶亚基I（COI）序列多年来一直被用作丽蝇科昆虫鉴定中合适的DNA标记；然而，使用短序列来确定亲缘关系较近的物种存在局限性。在本研究中，COI长序列被用于物种水平的鉴定。从中国22个省份的27个地点收集了72个标本，并明确鉴定为丽蝇科7个属的16个物种。对从中国内陆地区收集的与法医相关的丽蝇的线粒体COI长序列（1021 - 1382 bp）数据进行分析，为准确鉴定提供了可靠的标记。我们的数据为全球法医相关丽蝇物种鉴定提供了遗传多样性和参考，并对中国丽蝇在法医昆虫学实践中的未来利用具有指导意义。
