González-Jartı N Jesús M, Alfonso Amparo, Sainz María J, Vieytes Mercedes R, Botana Luis M
Departamento de Farmacología, Facultad de Veterinaria, Universidade de Santiago de Compostela , 27002 Lugo, Spain.
Departamento de Producción Vegetal, Facultad de Veterinaria, Universidade de Santiago de Compostela , 27002 Lugo, Spain.
J Agric Food Chem. 2017 Jun 14;65(23):4843-4852. doi: 10.1021/acs.jafc.7b01845. Epub 2017 Jun 5.
A method based on the combined use of ultraperformance liquid chromatography coupled to mass spectrometry-ion trap-time-of-flight (UPLC-MS-IT-TOF) detection was employed to identify the metabolite production of Aspergillus ochraceus, which is the major cause of food and feed contamination due to ochratoxin A. Under the proposed chromatographic conditions, seven metabolites belonging to the family of circumdatins were separated and identified. Their initial identification was performed through the exact molecular formula, as a function of their accurate mass. Collision-induced dissociation was applied to predict precursor and product ions, and the elemental composition of each compound was obtained. The elimination of nitrogenous groups followed by successive losses of carbonyl groups is the common fragmentation pathway of circumdatins. With the fragmentation data obtained, an UPLC-MS/MS method was created and optimized to detect circumdatins in corn samples.
采用一种基于超高效液相色谱-质谱联用-离子阱-飞行时间（UPLC-MS-IT-TOF）检测的方法来鉴定赭曲霉的代谢产物，赭曲霉是导致食品和饲料被赭曲霉毒素A污染的主要原因。在所提出的色谱条件下，分离并鉴定了七种属于环孢菌素家族的代谢产物。它们的初步鉴定是通过精确的分子式进行的，分子式是其精确质量的函数。应用碰撞诱导解离来预测前体离子和产物离子，并获得每种化合物的元素组成。环孢菌素的常见裂解途径是先消除含氮基团，随后连续失去羰基。利用获得的裂解数据，创建并优化了一种UPLC-MS/MS方法来检测玉米样品中的环孢菌素。
