Gudowska Monika, Gindzienska-Sieskiewicz Ewa, Gruszewska Ewa, Cylwik Bogdan, Sierakowski Stanislaw, Szmitkowski Maciej, Chrostek Lech
Medical University of Białystok, Department of Biochemical Diagnostics, Białystok, Poland.
Medical University of Białystok, Department of Rheumatology and Internal Diseases, Białystok, Poland.
Rev Bras Reumatol Engl Ed. 2017 May-Jun;57(3):185-189. doi: 10.1016/j.rbre.2016.01.005. Epub 2016 Mar 28.
The aim of this study was to assess the relationship between the two types of posttranslational modifications of proteins in RA: glycosylation on the example of carbohydrate-deficient transferrin and citrullination by means of autoantibodies to cyclic citrullinated peptides.
The study was carried out in 50 RA patients. CDT was measured using N Latex CDT immunonephelometric test, the results were presented in absolute and relative units. Anti-CCP were measured using the chemiluminescent method and rheumatoid factor by immunoturbidimetric method.
80% of RA patients were positive for anti-CCP, 70% for RF and 62% for both, anti-CCP and RF. The level of %CDT was significantly elevated, but absolute CDT level was not changed. The mean absolute CDT concentration was higher in anti-CCP positive patients than that in anti-CCP negative. CDT (absolute and relative concentration) did not correlate with anti-CCP and RF. However, serum RF significantly correlated with anti-CCP. %CDT did not correlate with anti-CCP, but absolute level correlated with anti-CCP only in anti-CCP negative and RF negative patients. CDT did not correlate with RF, but solely with anti-CCP in anti-CCP negative patients. Anti-CCP correlated with DAS 28 only in anti-CCP negative RA, but CDT (absolute and relative units) correlated with DAS 28 in all patients and in anti-CCP positive RA.
These results suggest that the changes in CDT and anti-CCP concentrations are not associated with oneself and indicate on the independence of these posttranslational modifications in rheumatoid arthritis. Only the alterations in transferrin glycosylation reflected the activity of RA.
本研究旨在通过以糖缺乏转铁蛋白为例的糖基化以及针对环瓜氨酸肽的自身抗体检测瓜氨酸化，评估类风湿关节炎（RA）中蛋白质的两种翻译后修饰之间的关系。
对50例RA患者进行了研究。采用N Latex CDT免疫比浊法检测CDT，结果以绝对单位和相对单位表示。采用化学发光法检测抗环瓜氨酸肽抗体（anti-CCP），采用免疫比浊法检测类风湿因子（RF）。
80%的RA患者anti-CCP呈阳性，70%的患者RF呈阳性，62%的患者anti-CCP和RF均呈阳性。%CDT水平显著升高，但绝对CDT水平未改变。anti-CCP阳性患者的平均绝对CDT浓度高于anti-CCP阴性患者。CDT（绝对浓度和相对浓度）与anti-CCP和RF均无相关性。然而，血清RF与anti-CCP显著相关。%CDT与anti-CCP无相关性，但仅在anti-CCP阴性和RF阴性患者中，绝对水平与anti-CCP相关。CDT与RF无相关性，但仅在anti-CCP阴性患者中与anti-CCP相关。anti-CCP仅在anti-CCP阴性的RA患者中与疾病活动度评分（DAS 28）相关，但CDT（绝对单位和相对单位）在所有患者以及anti-CCP阳性的RA患者中均与DAS 28相关。
这些结果表明，CDT和anti-CCP浓度的变化彼此不相关，提示类风湿关节炎中这些翻译后修饰具有独立性。只有转铁蛋白糖基化的改变反映了RA的活动度。
