Das Tulika, Kolli Vidyalatha, Karmakar Srijeeb, Sarkar Nandini
Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Odisha 769008, India.
Biomedicines. 2017 May 3;5(2):19. doi: 10.3390/biomedicines5020019.
Protein amyloids are characterized by aggregates that usually consist of fibres containing misfolded proteins and having a cross β-sheet conformation. These aggregates can eventually lead to several degenerative diseases like Alzheimer's disease, amyotrophic lateral sclerosis (ALS), Huntington's disease and Parkinson's disease. The present study describes the effect of chemically synthesized polyvinylpyrrolidone (PVP)-conjugated gold nanoparticles (PVP-AuNps) on hen egg white lysozyme (HEWL) amyloids. The synthesized nanoparticles have been characterized using various biophysical techniques like Ultraviolet-Visible (UV-Vis) Spectroscopy, Transmission electron microscopy (TEM), X-ray diffraction (XRD) analysis, dynamic light scattering (DLS), zeta-potential measurement and Fourier transform infrared spectroscopy (FTIR). The aggregation studies showed that PVP acts as a partial inhibitor of HEWL amyloidogenesis. However, when conjugated to gold nanoparticle surface, it leads to complete inhibition of amyloid formation. Apart from inhibition, PVP-conjugated gold nanoparticles also exhibited a significant disaggregation effect on mature amyloids and hence can be exploited as an effective therapeutic agent against hereditary systemic amyloidosis.
蛋白质淀粉样蛋白的特征是聚集体，通常由含有错误折叠蛋白质且具有交叉β-折叠构象的纤维组成。这些聚集体最终可导致多种退行性疾病，如阿尔茨海默病、肌萎缩侧索硬化症（ALS）、亨廷顿舞蹈症和帕金森病。本研究描述了化学合成的聚乙烯吡咯烷酮（PVP）共轭金纳米颗粒（PVP-AuNps）对鸡蛋清溶菌酶（HEWL）淀粉样蛋白的影响。已使用各种生物物理技术对合成的纳米颗粒进行了表征，如紫外可见（UV-Vis）光谱、透射电子显微镜（TEM）、X射线衍射（XRD）分析、动态光散射（DLS）、zeta电位测量和傅里叶变换红外光谱（FTIR）。聚集研究表明，PVP作为HEWL淀粉样蛋白生成的部分抑制剂。然而，当与金纳米颗粒表面共轭时，它会导致淀粉样蛋白形成的完全抑制。除了抑制作用外，PVP共轭金纳米颗粒对成熟淀粉样蛋白也表现出显著的解聚作用，因此可作为治疗遗传性全身性淀粉样变性的有效治疗剂。
