山梨糖对构巢曲霉产生NAD(P)酶的影响。

The effect of sorbose on NAD(P)ase production by Aspergillus nidulans.

da-Silva R, Jorge J A

Departmento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Brasil.

Braz J Med Biol Res. 1988;21(4):735-45.

Abstract

NAD(P)ase activity was stimulated when 1% sorbose was present in the culture medium of A. nidulans, and this effect was partially reversed by 1% glucose. 2. The level of extracellular NAD(P)ase was more affected by sorbose in the culture medium than the intracellular enzyme and no morphological changes were obtained. 3. The sorbose effect on NAD(P)ase activity appears to be specific since two other exoenzymes tested (beta-glucosidase and alkaline protease) show normal secretion patterns. 4. These findings suggest that the sorbose effect on NAD(P)ase production may be the consequence of metabolic disorders not necessarily linked with the morphological changes induced by the ketohexose.

摘要

当在构巢曲霉的培养基中存在1%山梨糖时，NAD(P)酶活性受到刺激，并且这种效应被1%葡萄糖部分逆转。2. 培养基中的山梨糖对细胞外NAD(P)酶水平的影响比对细胞内酶的影响更大，并且未观察到形态变化。3. 山梨糖对NAD(P)酶活性的影响似乎是特异性的，因为所测试的其他两种胞外酶（β-葡萄糖苷酶和碱性蛋白酶）显示出正常的分泌模式。4. 这些发现表明，山梨糖对NAD(P)酶产生的影响可能是代谢紊乱的结果，不一定与己酮糖诱导的形态变化有关。