地方性伯基特淋巴瘤患者中白细胞介素-10和CD4+CD25hi+调节性T细胞水平升高
High Levels of IL-10 and CD4+CD25hi+ Treg Cells in Endemic Burkitt's Lymphoma Patients.
作者信息
Futagbi Godfred, Gyan Ben, Nunoo Harriet, Tetteh John K A, Welbeck Jennifer E, Renner Lorna Awo, Ofori Michael, Dodoo Daniel, Edoh Dominic A, Akanmori Bartholomew D
机构信息
Department of Animal Biology and Conservation Science, College of Basic and Applied Sciences, University of Ghana, Legon, Accra, Ghana.
Immunology Department, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Accra, Ghana.
出版信息
Biomedicines. 2015 Aug 4;3(3):224-236. doi: 10.3390/biomedicines3030224.
BACKGROUND
The interplay between Epstein-Barr virus infection, malaria, and endemic Burkitt's Lymphoma is not well understood. Reports show diminished EBV-specific Th1 responses in children living in malaria endemic areas and deficiency of EBNA1-specific IFN-γ T cell responses in children with endemic Burkitt's Lymphoma (eBL). This study, therefore, examined some factors involved in the loss of EBNA-1-specific T cell responses in eBL.
METHODS
T-cell subset frequencies, activation, and IFN-γ- or IL-4-specific responses were analyzed by flow-cytometry. Plasma cytokine levels were measured by ELISA.
RESULTS
CD4+ and CD8+ cells in age- and sex-matched healthy controls ( = 3) expressed more IFN-γ in response to all immunostimulants than in pediatric endemic BL (eBL) patients ( = 4). In healthy controls, IFN-γ expression was higher than IL-4 expression, whereas in eBL patients the expression of IL-4 by CD4+ cells to EBNA-1 was slightly higher than IFN-γ. Moreover, the blood levels of TNF-α was significantly lower ( = 0.004) while IL-10 was significantly higher ( = 0.038), in eBL patients ( = 21) compared to controls ( = 16). Additionally, the frequency of CD4+CD25hi+ T cells was higher in both age-matched acute uncomplicated malaria ( = 26) and eBL ( = 14) patients compared to healthy controls ( = 19; = 0.000 and = 0.027, respectively).
CONCLUSION
The data suggest that reduced Th1 response in eBL might be due to increased levels of IL-10 and T reg cells.
背景
爱泼斯坦 - 巴尔病毒感染、疟疾与地方性伯基特淋巴瘤之间的相互作用尚未完全明确。报告显示，生活在疟疾流行地区的儿童中，EBV特异性Th1反应减弱，而地方性伯基特淋巴瘤（eBL）患儿中EBNA1特异性IFN - γ T细胞反应存在缺陷。因此，本研究探讨了一些与eBL中EBNA - 1特异性T细胞反应丧失有关的因素。
方法
通过流式细胞术分析T细胞亚群频率、活化情况以及IFN - γ或IL - 4特异性反应。采用酶联免疫吸附测定法检测血浆细胞因子水平。
结果
在年龄和性别匹配的健康对照者（n = 3）中，CD4⁺和CD8⁺细胞对所有免疫刺激剂产生的IFN - γ表达均高于儿科地方性伯基特淋巴瘤（eBL）患者（n = 4）。在健康对照者中，IFN - γ表达高于IL - 4表达，而在eBL患者中，CD4⁺细胞对EBNA - 1产生的IL - 4表达略高于IFN - γ。此外，与对照组（n = 16）相比，eBL患者（n = 21）的血液中TNF - α水平显著降低（P = 0.004），而IL - 10水平显著升高（P = 0.038）。另外，与健康对照者（n = 19）相比，年龄匹配的急性非复杂性疟疾患者（n = 26）和eBL患者（n = 14）中CD4⁺CD25hi⁺ T细胞的频率更高（分别为P = 0.000和P = 0.027）。
结论
数据表明，eBL中Th1反应降低可能是由于IL - 10水平升高和调节性T细胞增多所致。
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