Department of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu University, 1-1, Yanagido, Gifu, Gifu, 501-1193, Japan.
Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1, Yanagido, Gifu, Gifu, 501-1193, Japan.
Sci Rep. 2017 May 23;7(1):2301. doi: 10.1038/s41598-017-02607-1.
Circulating microRNAs in the blood may provide diagnostic and prognostic information about canine neoplastic diseases, and their profiles may be conserved between human and canine species. We performed RT-qPCR to obtain the profiles of circulating plasma microRNA-214 and -126 in total 181 cases of canine neoplastic diseases and healthy controls. MicroRNA-214 levels were high in 2 epithelial tumours (thyroid and mammary carcinomas) and 4 non-epithelial tumours (osteosarcoma, histiocytic sarcoma, chondrosarcoma, and hemangiosarcoma). In contrast, microRNA-126 levels were high in 6 epithelial tumours (mammary, hepatocellular, squamous cell, thyroid, transitional cell carcinomas, and adenocarcinoma) and 4 non-epithelial tumours (osteosarcoma, mast cell tumour, melanoma, and hemangiosarcoma). The diagnostic potential of microRNA-214 was relatively high in sarcomas, whereas that of microR-126 was high in most types of the tumours. MicroRNA-214 and -126 were prognostic predictors in 2 groups (adenocarcinoma and non-epithelial tumours except for osteosarcoma) and 3 groups (epithelial tumours, adenocarcinoma, and melanoma), respectively. Additionally, the microRNA levels did not show a strong correlation with the other clinical parameters. In conclusion, circulating microRNA-214 and -126 have the potential to be diagnostic and prognostic biomarkers for canine neoplastic diseases. Furthermore, their profiles may be key references as well for exploring novel biomarkers for human cancers.
循环 microRNA 可能为犬肿瘤疾病提供诊断和预后信息，且其谱在人与犬之间可能是保守的。我们进行了 RT-qPCR 以获得 181 例犬肿瘤病例和健康对照者的循环血浆 microRNA-214 和 -126 图谱。microRNA-214 在 2 种上皮肿瘤（甲状腺癌和乳腺癌）和 4 种非上皮肿瘤（骨肉瘤、组织细胞肉瘤、软骨肉瘤和血管肉瘤）中水平较高。相比之下，microRNA-126 在 6 种上皮肿瘤（乳腺、肝细胞癌、鳞状细胞癌、甲状腺癌、移行细胞癌和腺癌）和 4 种非上皮肿瘤（骨肉瘤、肥大细胞瘤、黑色素瘤和血管肉瘤）中水平较高。microRNA-214 在肉瘤中的诊断潜力相对较高，而 microR-126 在大多数类型的肿瘤中都具有较高的诊断潜力。microRNA-214 和 -126 分别是 2 组（腺癌和除骨肉瘤外的非上皮肿瘤）和 3 组（上皮肿瘤、腺癌和黑色素瘤）的预后预测因子。此外，microRNA 水平与其他临床参数之间没有很强的相关性。总之，循环 microRNA-214 和 -126 有可能成为犬肿瘤疾病的诊断和预后生物标志物。此外，其图谱也可能成为探索人类癌症新型生物标志物的关键参考。
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