Effects of NHCL application and removal on astrocytes and endothelial cells.

BACKGROUND

Hepatic encephalopathy (HE) is a complex disorder associated with increased ammonia levels in the brain. Although astrocytes are believed to be the principal cells affected in hyperammonemia (HA), endothelial cells (ECs) may also play an important role by contributing to the vasogenic effect of HA.

METHODS

Following acute application and removal of NHCl on astrocytes and endothelial cells, we analyzed pH changes, using fluorescence imaging with BCECF/AM, and changes in intracellular Ca concentration ([Ca]), employing fluorescence imaging with Fura-2/AM. Using confocal microscopy, changes in cell volume were observed accompanied by changes of [Ca] in astrocytes and ECs.

RESULTS

Exposure of astrocytes and ECs to 1 - 20 mM NHCl resulted in rapid concentration-dependent alkalinization of cytoplasm followed by slow recovery. Removal of the NHCl led to rapid concentration-dependent acidification, again followed by slow recovery. Following the application of NHCl, a transient, concentration-dependent rise in [Ca] in astrocytes was observed. This was due to the release of Ca from intracellular stores, since the response was abolished by emptying intracellular stores with thapsigargin and ATP, and was still present in the Ca-free bathing solution. The removal of NHCl also led to a transient concentration-dependent rise in [Ca] that resulted from Ca release from cytoplasmic proteins, since removing Ca from the bathing solution and emptying intracellular Ca stores did not eliminate the rise. Similar results were obtained from experiments on ECs. Following acute application and removal of NHCl no significant changes in astrocyte volume were detected; however, an increase of EC volume was observed after the administration of NHCl, and EC shrinkage was demonstrated after the acute removal of NHCl.

CONCLUSIONS

This study reveals new data which may give a more complete insight into the mechanism of development and treatment of HE.