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鉴定人类醛酮还原酶AKR7A2为一种新型的细胞珠蛋白结合伴侣。

The identification of human aldo-keto reductase AKR7A2 as a novel cytoglobin-binding partner.

作者信息

Li Xin, Zou Shanshan, Li Zhen, Cai Gaotai, Chen Bohong, Wang Ping, Dong Wenqi

机构信息

Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515 China.

出版信息

Cell Mol Biol Lett. 2016 Oct 24;21:25. doi: 10.1186/s11658-016-0026-9. eCollection 2016.

DOI:10.1186/s11658-016-0026-9
PMID:28536627
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5415844/
Abstract

Cytoglobin (CYGB), a member of the globin family, is thought to protect cells from reactive oxygen and nitrogen species and deal with hypoxic conditions and oxidative stress. However, its molecular mechanisms of action are not clearly understood. Through immunoprecipitation combined with a two-dimensional electrophoresis-mass spectrometry assay, we identified a CYGB interactor: aldo-keto reductase family 7 member A2 (AKR7A2). The interaction was further confirmed using yeast two-hybrid and co-immunoprecipitation assays. Our results show that AKR7A2 physically interacts with CYGB.

摘要

细胞红蛋白（CYGB）是珠蛋白家族的成员之一，被认为可保护细胞免受活性氧和氮物种的侵害，并应对缺氧条件和氧化应激。然而，其分子作用机制尚不清楚。通过免疫沉淀结合二维电泳-质谱分析，我们鉴定出一种CYGB相互作用分子：醛糖酮还原酶家族7成员A2（AKR7A2）。使用酵母双杂交和免疫共沉淀分析进一步证实了这种相互作用。我们的结果表明，AKR7A2与CYGB存在物理相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ac/5415844/e44e333b4bb3/11658_2016_26_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ac/5415844/30f974c60dcb/11658_2016_26_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ac/5415844/d9c9036c5c46/11658_2016_26_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ac/5415844/63f9142cb54e/11658_2016_26_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ac/5415844/e44e333b4bb3/11658_2016_26_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ac/5415844/30f974c60dcb/11658_2016_26_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ac/5415844/d9c9036c5c46/11658_2016_26_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ac/5415844/63f9142cb54e/11658_2016_26_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88ac/5415844/e44e333b4bb3/11658_2016_26_Fig4_HTML.jpg

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