Sarks Cory, Jin Mingjie, Balan Venkatesh, Dale Bruce E
Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, 3815 Technology Boulevard, Lansing, MI, 48910, USA.
DOE Great Lakes Bioenergy Research Center (GLBRC), Michigan State University, East Lansing, MI, 48824, USA.
J Ind Microbiol Biotechnol. 2017 Sep;44(9):1261-1272. doi: 10.1007/s10295-017-1949-5. Epub 2017 May 23.
The Rapid Bioconversion with Integrated recycling Technology (RaBIT) process uses enzyme and yeast recycling to improve cellulosic ethanol production economics. The previous versions of the RaBIT process exhibited decreased xylose consumption using cell recycle for a variety of different micro-organisms. Process changes were tested in an attempt to eliminate the xylose consumption decrease. Three different RaBIT process changes were evaluated in this work including (1) shortening the fermentation time, (2) fed-batch hydrolysate addition, and (3) selective cell recycling using a settling method. Shorting the RaBIT fermentation process to 11 h and introducing fed-batch hydrolysate addition eliminated any xylose consumption decrease over ten fermentation cycles; otherwise, decreased xylose consumption was apparent by the third cell recycle event. However, partial removal of yeast cells during recycle was not economical when compared to recycling all yeast cells.
集成回收技术快速生物转化（RaBIT）工艺利用酶和酵母回收来提高纤维素乙醇生产的经济性。RaBIT工艺的先前版本在使用细胞循环时，对于多种不同微生物而言，木糖消耗量有所下降。为消除木糖消耗量的下降，对工艺变化进行了测试。在这项工作中评估了三种不同的RaBIT工艺变化，包括（1）缩短发酵时间，（2）分批补料添加水解产物，以及（3）使用沉降法进行选择性细胞回收。将RaBIT发酵过程缩短至11小时并引入分批补料添加水解产物，在十个发酵周期内消除了木糖消耗量的任何下降；否则，到第三次细胞循环时，木糖消耗量下降就很明显了。然而，与回收所有酵母细胞相比，在循环过程中部分去除酵母细胞并不经济。
