Kumar Smita S, Malyan Sandeep K, Basu Suddhasatwa, Bishnoi Narsi R
Department of Environmental Science and Engineering, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, 125001, India.
Centre for Environment Science and Climate Resilient Agriculture, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Environ Sci Pollut Res Int. 2017 Jul;24(19):16019-16030. doi: 10.1007/s11356-017-9112-4. Epub 2017 May 23.
Anode chamber of a dual chamber microbial fuel cell (MFC) having raw landfill leachate was inoculated with consortium of sulphate-reducing bacteria (SRB) and sulphide-oxidizing bacteria (SOB) to study the phylogenetic architecture, function and mutualism of anolyte community developed in the reactor. Enriched microbial community was analysed with the help of Illumina MiSeq and indicated the dominance of Firmicutes (41.4%), Clostridia (36.4%) and Clostridium (12.9%) at phylum, class and genus level, respectively. Clostridium was associated with fermentation as well as transfer of electrons to the electrode mediated by ferredoxin. Desulfovibrio (6.7%), Aeromonas (6.6%) and Tetrathiobacter (9.8%) were SRB-SOB associated with direct electron transfer to the electrode. Community analysis disclosed a syntrophic association among novel Firmicutes and Proteobacteria species for bioelectricity generation and degradation of organic matter. Complete removal of chemical oxygen demand was observed from landfill leachate within 3 days of inoculation. Lower oxidative slope and polarization resistance revealed from Tafel analysis backed the feasibility of electron transfer from microbes to anodic electrode and thus development of efficient anode-respiring community. Following enrichment and stabilization of the anodic community, maximum power density achieved was 9.15 W/m and volumetric current density was 16.17 A/m. Simultaneous feeding with SRB-SOB and landfill leachate led to the enrichment of a novel, mutually interdependent microbial community capable of synchronized bioremediation of effluents rich in carbon, sulphate, nitrate and aromatic compounds.
在双室微生物燃料电池（MFC）的阳极室中加入未经处理的垃圾渗滤液，并接种硫酸盐还原菌（SRB）和硫化物氧化菌（SOB）的混合菌群，以研究该反应器中阳极电解液群落的系统发育结构、功能及共生关系。借助Illumina MiSeq对富集的微生物群落进行分析，结果表明，在门、纲和属水平上，厚壁菌门（41.4%）、梭菌纲（36.4%）和梭菌属（12.9%）分别占主导地位。梭菌属与发酵以及通过铁氧化还原蛋白介导的电子向电极转移有关。脱硫弧菌属（6.7%）、气单胞菌属（6.6%）和硫杆菌属（9.8%）是与直接电子转移至电极相关的硫酸盐还原菌-硫化物氧化菌。群落分析揭示了新型厚壁菌门和变形菌门物种之间在生物电产生和有机物降解方面的互营关系。接种后3天内，垃圾渗滤液中的化学需氧量被完全去除。塔菲尔分析显示的较低氧化斜率和极化电阻支持了微生物向阳极电极进行电子转移的可行性，进而支持了高效阳极呼吸群落的形成。阳极群落富集并稳定后，实现的最大功率密度为9.15 W/m，体积电流密度为16.17 A/m。同时向系统中加入硫酸盐还原菌-硫化物氧化菌和垃圾渗滤液，导致富集了一个新型的、相互依存的微生物群落，该群落能够同步生物修复富含碳、硫酸盐、硝酸盐和芳香族化合物的废水。
