Akiyoshi H
Department of Health Sciences, Faculty of Integrated Arts and Sciences, University of Tokushima.
J Biochem. 1988 Oct;104(4):521-5. doi: 10.1093/oxfordjournals.jbchem.a122503.
Three kinds of deoxyribonucleases (peaks A, B, and C) were separated from purified rat liver nuclei on DEAE-cellulose column chromatography, and their characteristics were partially studied. Peaks A and B had endonuclease activities under acidic conditions with low substrate specificity and did not require divalent metal ions. Peak C had an exonuclease activity under alkaline conditions with substrate specificity for denatured DNA or single stranded homopolymer and required divalent cations. Peak C degraded 3'-terminally mismatched substrate much faster than 3'-terminally matched substrate.
通过DEAE - 纤维素柱层析从纯化的大鼠肝细胞核中分离出三种脱氧核糖核酸酶（峰A、峰B和峰C），并对它们的特性进行了部分研究。峰A和峰B在酸性条件下具有内切酶活性，底物特异性低，且不需要二价金属离子。峰C在碱性条件下具有外切酶活性，对变性DNA或单链均聚物具有底物特异性，并且需要二价阳离子。峰C降解3'-末端错配的底物比3'-末端匹配的底物快得多。
