DNA中对脱嘌呤位点具有特异性的大肠杆菌核酸内切酶与核酸外切酶III之间的关系。
Relation between Escherichia coli endonucleases specific for apurinic sites in DNA and exonuclease III.
作者信息
Ljungquist S, Lindahl T
出版信息
Nucleic Acids Res. 1977 Aug;4(8):2871-9. doi: 10.1093/nar/4.8.2871.
Abstract
Contradictory data have recently been published from two different laboratories on the presence vs absence of an intrinsic endonucliolytic activity of E. coli exonuclease III at apurinic sites in double-stranded DNA. It is shown here that an endonuclease activity of this specificity co-chromatographs exactly with exonuclease III on phosphocellulose and Sephadex G-75 columns, indicating that the endonuclease and exonuclease activities are due to the same enzyme. In addition, another E. coli endonuclease specific for apurinic sites exists, which can be separated from exonuclease III by the same chromatographic procedures.
摘要
最近，两个不同实验室公布了相互矛盾的数据，涉及大肠杆菌核酸外切酶III在双链DNA的脱嘌呤位点是否存在内在内切核酸酶活性。本文表明，这种特异性的内切酶活性在磷酸纤维素和葡聚糖G-75柱上与核酸外切酶III完全共色谱，表明内切酶和核酸外切酶活性是由同一种酶引起的。此外，还存在另一种对脱嘌呤位点具有特异性的大肠杆菌内切酶，它可以通过相同的色谱方法与核酸外切酶III分离。
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本文引用的文献
1
2
3
Endonuclease II of Escherichia coli. Degradation of partially depurinated deoxyribonucleic acid.大肠杆菌的核酸内切酶II。部分脱嘌呤脱氧核糖核酸的降解
Biochemistry. 1971 Dec 21;10(26):4986-93. doi: 10.1021/bi00802a024.
4
Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.脱氧核糖核酸的酶促合成。二十六。一种纯一的脱氧核糖核酸聚合酶的物理和化学研究。
J Biol Chem. 1969 Jun 10;244(11):2996-3008.
5
Nuclease for DNA apurinic sites may be involved in the maintenance of DNA in normal cells.用于DNA脱嘌呤位点的核酸酶可能参与正常细胞中DNA的维持。
Nat New Biol. 1973 Jul 18;244(133):67-9. doi: 10.1038/newbio244067a0.
6
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7
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8
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9
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