Birbari A E, Hajj-Ali A F, Mukaddam-Daher S S, Jirjus A R
Division of Nephrology-Hypertension, American University Medical Center, Beirut, Lebanon.
J Hypertens Suppl. 1988 Dec;6(4):S264-5. doi: 10.1097/00004872-198812040-00080.
This study was designed to explore factors involved in the regulation of Na-K pumps in erythrocytes and blood pressure in anephric Sprague-Dawley female rats. Twenty-four hours following bilateral nephrectomy, the rats were given a 1-h infusion of 1 ml of either 0.9% or 5.4% saline, or 5% or 30% glucose. At the completion of the infusion, tail blood pressure was determined and blood was drawn for the measurement of the ouabain-furosemide-sensitive sodium efflux rate constant in erythrocytes. Infusion of 5.4% saline or 30% glucose caused a significant reduction in the erythrocyte sodium efflux rate constant, more pronounced with the former, and an elevation in blood pressure. Nephrectomy or infusion of 0.9% saline or 5% glucose had no effect on either parameter. These findings suggest that in anephric rats (1) extracellular fluid hyperosmolality rather than extracellular fluid volume expansion inhibits the Na-K pump in all cells; (2) a Na-K pump inhibitor is involved in the genesis of hypertension.
本研究旨在探讨无肾的Sprague-Dawley雌性大鼠红细胞中钠钾泵调节及血压调节的相关因素。双侧肾切除术后24小时，给大鼠输注1小时1毫升的0.9%或5.4%生理盐水，或5%或30%葡萄糖。输注结束时，测定尾血压并采血以测量红细胞中哇巴因-呋塞米敏感的钠外流速率常数。输注5.4%生理盐水或30%葡萄糖导致红细胞钠外流速率常数显著降低，前者更明显，并导致血压升高。肾切除或输注0.9%生理盐水或5%葡萄糖对这两个参数均无影响。这些发现表明，在无肾大鼠中：(1)细胞外液高渗而非细胞外液容量扩张抑制所有细胞中的钠钾泵；(2)一种钠钾泵抑制剂参与高血压的发生。
