Yang Rui, Liu Yuqian, Zhou Zhongkai, Sheng Jiping, Meng Demei
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology , Tianjin 300457, People's Republic of China.
Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center , Tianjin 300457, People's Republic of China.
J Agric Food Chem. 2017 Jun 14;65(23):4668-4675. doi: 10.1021/acs.jafc.7b00902. Epub 2017 Jun 5.
A homemade microcurrent reactor was used to treat the fermentation of Trichoderma reesei. Results indicated that the yield of saccharides for T. reesei RUT-C30 cultivated in pumpkin lignocellulose broth reaches 38.86% (w/w) when a microcurrent treatment (20 mA, at the 48th hour for 60 min) was carried out, which is significantly higher than the control group (p < 0.05). Additionally, activities of endoglucanase, cellobiohydrolase, xylanase, and pectinase were significantly increased in days 3-7. Furthermore, the fungal growth was facilitated by microelectric treatment, showing a 0.57-fold increase of spore numbers at the sixth day of cultivation. Besides, the monosaccharide composition, including glucose (1.03 mg/mL), xylose (0.12 mg/mL), arabinose (0.31 mg/mL), and fructose (0.13 mg/mL), extracted from the reactor was higher than that without the current treatment. In this work, we improved the biodegradation of lignocellulosic wastes by applying a microcurrent to lignocellulose-degrading fungal cultures and provided a new idea for the lignocellulose material pretreatment and bioconversion.
使用自制的微电流反应器处理里氏木霉的发酵过程。结果表明，当进行微电流处理（20 mA，在第48小时处理60分钟）时，在南瓜木质纤维素肉汤中培养的里氏木霉RUT - C30的糖类产量达到38.86%（w/w），这显著高于对照组（p < 0.05）。此外，在第3 - 7天内切葡聚糖酶、纤维二糖水解酶、木聚糖酶和果胶酶的活性显著增加。此外，微电处理促进了真菌生长，在培养的第六天孢子数增加了0.57倍。此外，从反应器中提取的单糖成分，包括葡萄糖（1.03 mg/mL）、木糖（0.12 mg/mL）、阿拉伯糖（0.31 mg/mL）和果糖（0.13 mg/mL），高于未进行电流处理的情况。在这项工作中，我们通过对木质纤维素降解真菌培养物施加微电流，改善了木质纤维素废物的生物降解，并为木质纤维素材料的预处理和生物转化提供了新思路。
