a Ocular Surface Research Group, Singapore Eye Research Institute, Singapore.
b Corneal and External Eye Disease Department, Singapore National Eye Center , Singapore.
Ocul Immunol Inflamm. 2018;26(7):1069-1077. doi: 10.1080/09273948.2017.1316411. Epub 2017 May 24.
To evaluate the basal and possibly stimulated expression of interleukin (IL)-17 in the context of the ocular surface and potential downstream effects.
Western blot and immunofluorescent staining were used to evaluate IL-17F expression in human cornea/conjunctival tissues and cornea epithelial cell line. Cytokines and matrix metalloproteinase (MMP) transcripts were quantified by qPCR. IL-17F effects on NF-κB were investigated by the secretary alkaline phosphatase assay.
IL-17F was expressed in the cytoplasm of human corneal and conjunctival epithelial tissues. In the corneal cell line, exogenous IL-17F did not increase the NF-κB activity, but Pam3CSK4 increased IL-17F transcripts. IL-17F stimulated MMP-9 activity, promoted IL-6, IL-8, tumor necrosis factor-α transcripts levels, and depressed monocyte chemoattractant protein-1 expression, but did not affect transforming growth factor beta-1 transcript levels.
Normal corneal/conjunctival epithelial cells express IL-17F. Microbial agents may stimulate IL-17F via the NF-κB pathway. Matrix dissolution stimulated by IL-17F may have a role in the melting and necrosis of cornea in severe inflammation.
评估眼表中白细胞介素（IL）-17 的基础表达和可能的刺激表达及其潜在的下游效应。
采用 Western blot 和免疫荧光染色法评估人角膜/结膜组织和角膜上皮细胞系中 IL-17F 的表达。通过 qPCR 定量细胞因子和基质金属蛋白酶（MMP）转录本。通过碱性磷酸酶分泌测定法研究 IL-17F 对 NF-κB 的影响。
IL-17F 表达于人角膜和结膜上皮组织的细胞质中。在角膜细胞系中，外源性 IL-17F 不会增加 NF-κB 活性，但 Pam3CSK4 会增加 IL-17F 转录本。IL-17F 刺激 MMP-9 活性，促进 IL-6、IL-8、肿瘤坏死因子-α 转录本水平，抑制单核细胞趋化蛋白-1 表达，但不影响转化生长因子-β-1 转录本水平。
正常角膜/结膜上皮细胞表达 IL-17F。微生物制剂可能通过 NF-κB 途径刺激 IL-17F。IL-17F 刺激的基质溶解可能在严重炎症中角膜融解和坏死中起作用。
