The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China.
Eur Rev Med Pharmacol Sci. 2017 May;21(9):2167-2171.
We conducted this study to analyze the mechanism behind the high coagulation state induced by circulating plasma microparticle tissue factor (MP-TF) in patients with breast cancer.
87 cases of breast cancer patients (10 cases of TNM stage I, 16 cases of II, 32 cases of III, 29 cases of IV; 8 cases of pathological type in situ carcinoma, 15 cases of ductal carcinoma, 64 cases of invasive cancer) were used as the observation group and 20 cases of benign breast lesions were used as the control group to compare MP-TF levels of plasma and coagulation parameters including prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB) and D-dimer body (D-D) level and NF-κB signaling pathway index including P50, p65, TAK1 and IκBα levels.
The plasma MP-TF level in the observation group was significantly higher than that in control group, and the level of MP-TF in the observation group increased with an increase in depth of TNM stage and tumor invasion; differences were statistically significant (p<0.05). In the observation group, the plasma PT and APTT were shortened, and the levels of FIB and D-D were increased; the differences were statistically significant (p<0.05). In the observation group, the levels of P50, p65, TAK1, IκBαin circulating blood were higher than those in control group; the differences were statistically significant (p<0.05). After the Pearson test, the plasma levels of MP-TF in patients with breast cancer were negatively correlated with PT and APTT, and positively correlated with FIB, D-D values and the levels of p50, p65, TAK1 and IκBα (4 p<0.05).
MP-TF can lead to high blood coagulation in patients with breast cancer through the activation of NF-κB signaling pathway, which may become a new target for the intervention of the disease.
本研究旨在分析循环血浆微颗粒组织因子（MP-TF）诱导乳腺癌患者高凝状态的机制。
将 87 例乳腺癌患者（10 例 TNM Ⅰ期，16 例Ⅱ期，32 例Ⅲ期，29 例Ⅳ期；原位癌 8 例，导管癌 15 例，浸润性癌 64 例）作为观察组，20 例良性乳腺病变患者作为对照组，比较两组血浆 MP-TF 水平及凝血参数，包括凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）、纤维蛋白原（FIB）、D-二聚体（D-D）水平，以及 NF-κB 信号通路指标，包括 P50、p65、TAK1 和 IκBα 水平。
观察组血浆 MP-TF 水平明显高于对照组，且观察组 MP-TF 水平随 TNM 分期和肿瘤浸润深度的增加而升高；差异有统计学意义（p<0.05）。观察组血浆 PT 和 APTT 缩短，FIB 和 D-D 水平升高；差异有统计学意义（p<0.05）。观察组外周血 P50、p65、TAK1、IκBα 水平高于对照组；差异有统计学意义（p<0.05）。Pearson 检验后，乳腺癌患者血浆 MP-TF 水平与 PT 和 APTT 呈负相关，与 FIB、D-D 值及 p50、p65、TAK1、IκBα 水平呈正相关（p<0.05）。
MP-TF 可通过激活 NF-κB 信号通路导致乳腺癌患者血液高凝，可能成为疾病干预的新靶点。
