miR-425-5p 通过调节 CYLD 在胃癌中促进肿瘤进展。
MiR-425-5p promotes tumor progression via modulation of CYLD in gastric cancer.
机构信息
General Surgery Department II, Chinese PLA General Hospital, Beijing, China.
出版信息
Eur Rev Med Pharmacol Sci. 2017 May;21(9):2130-2136.
OBJECTIVE
MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized as key players in carcinogenesis. The present study investigated the role of miR-425-5p in the development and progression of gastric cancer (GC).
PATIENTS AND METHODS
The miR-425-5p level in GC tissues and cells was assayed by qRT-PCR. Then, the effects of miR-425-5p expression on the biological behavior of GC cells were investigated. Analysis of target protein expression was determined by Western blotting. Bioinformatic prediction and luciferase assays were employed to identify the predicted miRNA which regulates CYLD.
RESULTS
miR-425-5p was found to be up-regulated in GC tissues and cell lines. Knockdown of miR-425-5p in GC cells attenuated migration and invasion of GC cells, whereas overexpression of miR-425-5p promoted cell migration and invasion. The luciferase assay demonstrated that CYLD was a direct target of miR-425-5p. Furthermore, the miR-425-5p level was inversely correlated with levels of CYLD in Western blotting assay.
CONCLUSIONS
Our findings indicate that miR-425-5p may contribute to the progression of GC through a mechanism involving CYLD, suggesting that miR-425-5p may have the potential to be a novel important alternative therapeutic target for GC.
目的
微小 RNA（miRNAs）已成为重要的基因调控因子，并被认为是癌症发生的关键因素。本研究探讨了 miR-425-5p 在胃癌（GC）发展和进展中的作用。
患者和方法
通过 qRT-PCR 检测 GC 组织和细胞中 miR-425-5p 的水平。然后，研究了 miR-425-5p 表达对 GC 细胞生物学行为的影响。通过 Western blot 分析靶蛋白表达。采用生物信息学预测和荧光素酶报告基因实验鉴定调节 CYLD 的预测 miRNA。
结果
miR-425-5p 在 GC 组织和细胞系中上调。GC 细胞中 miR-425-5p 的敲低减弱了 GC 细胞的迁移和侵袭，而 miR-425-5p 的过表达促进了细胞迁移和侵袭。荧光素酶报告基因实验表明 CYLD 是 miR-425-5p 的直接靶标。此外，Western blot 实验表明 miR-425-5p 水平与 CYLD 水平呈负相关。
结论
我们的研究结果表明，miR-425-5p 可能通过涉及 CYLD 的机制促进 GC 的进展，提示 miR-425-5p 可能成为 GC 新的重要治疗靶点。
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