Zhang Kejin, Guo Ling
Department of Dermatology, Liaocheng City People's Hospital, Liaocheng City, Shandong Province 252000, People's Republic of China.
Department of Dermatology, Liaocheng City People's Hospital, Liaocheng City, Shandong Province 252000, People's Republic of China.
Gene. 2018 Jan 30;641:272-278. doi: 10.1016/j.gene.2017.10.055. Epub 2017 Oct 18.
MicroRNAs (miRNAs) have emerged as critical regulators for cancer development and progression of human melanoma. However, the potential molecular mechanism of miR-767 in human melanoma has not been intensively investigated. In this present study, we confirmed that miR-767 was frequently up-regulated in human melanoma tissues and cell lines. Ectopic expression of miR-767 promoted cell proliferation in human melanoma cell lines A375 and WM35, whereas miR-767-in reversed the function. Bioinformatics analysis revealed that cylindromatosis (CYLD) was hypothesized to be a possible target gene of miR-767, and this was confirmed by luciferase activity assay. Knockdown of CYLD counteracted the proliferation arrest by miR-767-in in melanoma cells A375 and WM35. In conclusion, our study indicated that miR-767 acted as a role of tumor promoter by targeting CYLD in human melanoma, and might serve as a prognostic or therapeutic target for human melanoma.
微小RNA（miRNA）已成为人类黑色素瘤癌症发展和进展的关键调节因子。然而，miR-767在人类黑色素瘤中的潜在分子机制尚未得到深入研究。在本研究中，我们证实miR-767在人类黑色素瘤组织和细胞系中经常上调。miR-767的异位表达促进了人类黑色素瘤细胞系A375和WM35中的细胞增殖，而miR-767抑制剂则逆转了这一功能。生物信息学分析表明，圆柱瘤蛋白（CYLD）被推测为miR-767的一个可能靶基因，荧光素酶活性测定证实了这一点。在黑色素瘤细胞A375和WM35中，敲低CYLD可抵消miR-767抑制剂引起的增殖停滞。总之，我们的研究表明，miR-767通过靶向CYLD在人类黑色素瘤中发挥肿瘤促进作用，并可能作为人类黑色素瘤的预后或治疗靶点。
