College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Beijing Centre for Physical and Chemical Analysis, Beijing 100089, China.
Bioresour Technol. 2017 Sep;239:454-463. doi: 10.1016/j.biortech.2017.04.071. Epub 2017 Apr 21.
Although trace elements are known to aid anaerobic digestion, their mechanism of action is still unclear. High-throughput sequencing was used to reveal the mechanism by which adding trace elements affects microbial communities and their action. The results showed that the highest methane yields, with addition of Fe, Mo, Se and Mn were 289.2, 289.6, 285.3, 293.0mL/g volatile solids (VS), respectively. The addition of Fe, Mo, Se and Mn significantly (P<0.05) reduced the level of volatile fatty acids (VFAs). The dominant bacteria and archaea were Bacteroidetes and Methanosaeta, respectively. Compared with the proportion of Methanosaeta in the control group, treatment with added trace elements increased Methanosaeta by as much as 12.4%. Microbial community analysis indicated that adding trace elements changed the composition and diversity of archaea and bacteria. Methane yield was positively correlated with bacterial diversity and negatively correlated with archaeal diversity for most treatments.
虽然微量元素被认为有助于厌氧消化，但它们的作用机制仍不清楚。高通量测序被用来揭示添加微量元素影响微生物群落及其作用的机制。结果表明，添加 Fe、Mo、Se 和 Mn 时，甲烷产量最高，分别为 289.2、289.6、285.3 和 293.0mL/g 挥发性固体（VS）。添加 Fe、Mo、Se 和 Mn 显著（P<0.05）降低了挥发性脂肪酸（VFAs）的水平。优势细菌和古菌分别为拟杆菌门和产甲烷菌门。与对照组相比，添加微量元素的处理使产甲烷菌增加了 12.4%。微生物群落分析表明，添加微量元素改变了古菌和细菌的组成和多样性。对于大多数处理，甲烷产量与细菌多样性呈正相关，与古菌多样性呈负相关。
