Lowe J, Lennox G, Jefferson D, Morrell K, McQuire D, Gray T, Landon M, Doherty F J, Mayer R J
Department of Pathology, Nottingham University Medical School, Queens Medical Centre, U.K.
Neurosci Lett. 1988 Nov 22;94(1-2):203-10. doi: 10.1016/0304-3940(88)90296-0.
Using an immunocytochemical method to localise antibodies to ubiquitin, filamentous inclusion bodies were seen in spinal anterior horn neurones in cases of motor neurone disease (MND) but not in any control cases. These inclusion bodies appeared to be closely associated with classical Bunina bodies and immuno-electron microscopy suggested that they were based on arrays of straight 10-15 nm filaments together with some granular material. These observations link the protein ubiquitin with a chronic neurodegenerative disease and extend previous observations of a close association between filamentous inclusion bodies and ubiquitin. Ubiquitin-filament inclusions should be regarded as a new hallmark in the histological diagnosis of MND.
运用免疫细胞化学方法对泛素抗体进行定位，在运动神经元病（MND）患者的脊髓前角神经元中发现了丝状包涵体，而在任何对照病例中均未发现。这些包涵体似乎与典型的布宁纳小体密切相关，免疫电子显微镜显示它们基于10 - 15纳米的直丝阵列以及一些颗粒物质。这些观察结果将蛋白质泛素与一种慢性神经退行性疾病联系起来，并扩展了先前关于丝状包涵体与泛素密切关联的观察。泛素丝包涵体应被视为MND组织学诊断中的一个新标志。
