Lowe J, Aldridge F, Lennox G, Doherty F, Jefferson D, Landon M, Mayer R J
Department of Pathology, Nottingham University Medical School, Queens Medical Centre, U.K.
Neurosci Lett. 1989 Oct 23;105(1-2):7-13. doi: 10.1016/0304-3940(89)90003-7.
Histological sections of cerebral motor cortex, brainstem, and spinal cord from 10 cases of clinically diagnosed motor neurone disease (MND) and 10 control cases were examined by conventional histology and immunocytochemical methods to localise ubiquitin. Intracytoplasmic inclusion bodies were identified in motor neurones of hypoglossal nuclei and appeared specific for MND. Similar inclusions were found in both large pyramidal cells and small neurones in the motor cortex, and were restricted to 4 cases having the amyotrophic lateral sclerosis form of MND with severe degeneration of corticospinal tracts. As reported in earlier studies, cellular inclusion bodies were identified in motor neurones of spinal cord from cases of MND but not in control material. Ubiquitin inclusions in motor neurones appear to be markers for the degenerative process causing neuronal loss in MND and there appears to be a close association between the anatomical location of inclusions and clinical manifestations of disease.
采用传统组织学和免疫细胞化学方法，对10例临床诊断为运动神经元病（MND）患者及10例对照者的大脑运动皮质、脑干和脊髓组织切片进行检查，以定位泛素。在舌下神经核的运动神经元中发现了胞质内包涵体，这些包涵体似乎是MND所特有的。在运动皮质的大锥体细胞和小神经元中也发现了类似的包涵体，且仅在4例患有肌萎缩侧索硬化型MND且皮质脊髓束严重变性的病例中出现。如早期研究报道，在MND患者脊髓的运动神经元中发现了细胞包涵体，而对照材料中未发现。运动神经元中的泛素包涵体似乎是导致MND中神经元丢失的退行性过程的标志物，且包涵体的解剖位置与疾病临床表现之间似乎存在密切关联。
