Ríos-León Karla, Fuertes-Ruiton Cesar, Arroyo Jorge, Ruiz Julio
Instituto de Ciencias Farmacéuticas y Recursos Naturales, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos. Lima, Perú.
Instituto de Investigaciones Clínicas, Facultad de Medicina, Universidad Nacional Mayor de San Marcos. Lima, Perú.
Rev Peru Med Exp Salud Publica. 2017 Jan-Mar;34(1):70-75. doi: 10.17843/rpmesp.2017.341.2768.
To determine the toxicity and chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine (DMH).
The alkaloid extract was obtained from the fleshy part of M. bellavistensis, and an acute toxicity test was then carried out on 30 mice of the Balb C57 strain. To assess its chemoprotective effect, colon cancer was induced in 45 Holtzman rats using DMH according to the following experimental design: one control group received 2 mL/kg sodium polysorbate, and four groups received 20 mg/kg DMH plus 0, 1, 5, or 10 mg/kg M. bellavistensis alkaloid extract.
With a sample of 5 g of alkaloid extract, an LD50 greater than 1000 mg/mL was determined in the acute toxicity test. Histological indicators revealed that the 5 and 10 mg/kg doses had significant anti-tumor activity with 100% neoplasia inhibition against DMH- induced colon cancer in rats.
Under experimental conditions, the alkaloid extract of M. bellavistensis has a chemoprotective effect against DMH-induced colon cancer in rats.
确定美花角生物碱提取物对用1,2 - 二甲基肼（DMH）诱导的大鼠结肠癌的毒性和化学保护作用。
从美花角肉质部分获得生物碱提取物，然后对30只Balb C57品系小鼠进行急性毒性试验。为评估其化学保护作用，根据以下实验设计，用DMH诱导45只霍尔茨曼大鼠患结肠癌：一个对照组接受2 mL/kg聚山梨醇酯80，四个组接受20 mg/kg DMH加0、1、5或10 mg/kg美花角生物碱提取物。
在急性毒性试验中，用5 g生物碱提取物样本测定的半数致死量大于1000 mg/mL。组织学指标显示，5和10 mg/kg剂量具有显著的抗肿瘤活性，对DMH诱导的大鼠结肠癌的肿瘤形成抑制率达100%。
在实验条件下，美花角生物碱提取物对DMH诱导的大鼠结肠癌具有化学保护作用。
