[印加果油（星油藤）对1,2-二甲基肼诱导的霍尔兹曼大鼠结肠癌发生的影响]

[Effect of Sacha Inchi oil (Plukenetiavolubilis L.) on 1,2-dimethylhydrazine-induced colon carcinogenesis in Holtzman rats].

作者信息

Centurión-Rodríguez César Abel, Huamán-Saavedra Juan Jorge, Requena-Fuentes Víctor Raúl

机构信息

Facultad de Medicina, Universidad Nacional de Trujillo. Trujillo, Perú.

Facultad de Medicina, Universidad Nacional de Trujillo. Trujillo, Perú; Departamento de Patología, Hospital Belén. Trujillo, Perú.

出版信息

Rev Gastroenterol Peru. 2017 Apr-Jun;37(2):129-136.

PMID:28731992

Abstract

OBJECTIVES

To evaluate the preventive effect of Sacha Inchi oil (SIO) on 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis (CC) in Holtzman rats.

MATERIALS AND METHODS

Experimental study with 28 Holtzman male albino rats randomly distributed into 4 groups: a positive control group exposed to DMH (C1), a negative control group exposed to SIO at 150 uL/kg/day (C2), and two experimental groups exposed to DMH with SIO at 150 uL/kg/day (E1) and SIO at 300 uL/kg/day (E2). The DMH was applied for 8 weeks and the total induction time was 22 weeks. Pathological examination was performed by identifying cancerous tumor lesions in the guts. The preventive effect was evaluated based on proportions of lack of lesion in the groups exposed to DMH.

RESULTS

Cancerous tumor lesions were identified in: two specimens of group C1, one specimen of group E1 and two specimens of group E2. No intestinal lesions were identified in group C2. The proportions of lack of lesion were: in group C1 of 75%, in group E1 of 87.5% and group E2 of 75%. No significant differences were found (p>0.05).

CONCLUSIONS

It was not found a significant protective effect of SIO on DMH-induced CC in Holtzman rats, compared to control group.

摘要

目的

评估印加果油（SIO）对1,2 - 二甲基肼（DMH）诱导的霍尔兹曼大鼠结肠癌发生（CC）的预防作用。

材料与方法

对28只霍尔兹曼雄性白化大鼠进行实验研究，随机分为4组：暴露于DMH的阳性对照组（C1）、以150 μL/kg/天的剂量暴露于SIO的阴性对照组（C2），以及两个以150 μL/kg/天的剂量同时暴露于DMH和SIO的实验组（E1）和以300 μL/kg/天的剂量暴露于SIO的实验组（E2）。DMH给药8周，总诱导时间为22周。通过识别肠道中的癌性肿瘤病变进行病理检查。根据暴露于DMH的组中无病变的比例评估预防效果。